Anti-Muscarinic Acetylcholine Receptor m2 Antibody, clone M2-2-B3

m2 muscarinic acetylcholine receptor. No reactivity with the other subtypes.

SPECIES REACTIVITIES:

Expected to react with most mammalian species.

i3 loop of m2 receptor fusion protein (225-359), fused to Glutathione S-transferase.

Anti-Muscarinic Acetylcholine Receptor m2 Antibody, clone M2-2-B3 is an antibody against Muscarinic Acetylcholine Receptor m2 for use in IC, IH & IP.

Immunohistochemistry on 4% paraformaldehyde fixed tissue. Does not work on paraffin embedded tissues. Suggested starting concentration 1-5 μg/mL. It is suggested that you use the PAP system if using this antibody on rat. Immunocytochemistry on transfected cells

Immunoprecipitation Works poorly for immunoblotting Optimal working dilutions must be determined by end user.

IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB367

This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.

1) 3 x 10 minute washes in TBS (with or without 0.25% Triton).

2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).

3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C.

4) 3 x 10 minute washes in TBS.

5) Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody).

6) 3 x 10 minute washes in TBS.

7) ABC Elite (1:200 Vector Labs) in TBS.

8) 2 x 10 minute washes in TBS.

9) 1 x 10 minute wash in phosphate buffer (no saline).

10) DAB reaction with 0.06% NiCl added for intensification.

11) 2 x 10 minute washes in PBS.

12) 1 x 10 minute wash in phosphate buffer (no saline).

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Format: Purified

Purified immunoglobulin. Liquid in 0.02 M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide, pH 7.6.

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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