MAB3785
Anti-APC Antibody, NT, clone Ali 12.28
clone Ali 12.28, Chemicon®, from mouse
Synonym(s):
Adenomatous Polyposis Coli Protein
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
Ali 12.28, monoclonal
Application:
ELISA, IHC, IP, WB
Citations:
1
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
Ali 12.28, monoclonal
species reactivity
human
should not react with
mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... APC(324)
mouse ... Apc(11789)
General description
The adenomatous polyposis coli tumor suppressor gene is mutated (often deletion of the C-terminal portion of APC) in the inherited disease, familial adenomatous polyposis (FAP), and over 80% of colorectal cancers. Ali 12-28 can be used for APC expression and detection of APC mutations.
Immunogen
Epitope: N-terminus
N-terminal APC-MBP fusion protein (first 1.3 kb of APC which includes the first 433 aa and the first 9 exons of the gene).
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Use Anti-APC Antibody, N-terminus, clone Ali 12.28 (Mouse Monoclonal Antibody) validated in ELISA, IP, WB, IHC to detect APC also known as Adenomatous Polyposis Coli Protein.
Western blot: 4 μg/mL
Immunohistochemistry (Acetone Fixed, Frozen)
ELISA
Immunoprecipitation
Optimal working dilutions must be determined by end user.
Immunohistochemistry (Acetone Fixed, Frozen)
ELISA
Immunoprecipitation
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Recognizes human APC, molecular weight ~310 kDa, as well as C-terminal truncation mutants containing the epitope mapped between amino acids 135 to 422.
Physical form
Format: Purified
Purified immunoglobulin. Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide.
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.
Analysis Note
Control
POSITIVE CONTROL: SW480, LoVo, DLD1, and SW837 cell lines
POSITIVE CONTROL: SW480, LoVo, DLD1, and SW837 cell lines
Other Notes
Replaces: AB1268
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Storage Class
12 - Non Combustible Liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Jie Zhong et al.
Nature communications, 13(1), 4961-4961 (2022-08-25)
The adenomatous polyposis coli (APC)-Rho guanine nucleotide exchange factor 4 (Asef) protein-protein interaction (PPI) is essential for colorectal cancer metastasis, making it a promising drug target. Herein, we obtain a sensitivity-enhanced tracer (tracer 7) with a high binding affinity (Kd = 0.078 μM)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB3785 | 04053252666544 |