MAB3878
Anti-MyoD1 Antibody
clone 5.2F, Chemicon®, from mouse
Synonym(s):
Anti-MYF3, Anti-MYOD, Anti-MYODRIF, Anti-PUM, Anti-bHLHc1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
5.2F, monoclonal
Application:
ICC, IHC, IP, WB
Citations:
8
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
5.2F, monoclonal
species reactivity
rat, mouse, chicken, human
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MYOD1(4654)
General description
~ 45 kDa
Immunogen
Recombinant protein from mouse MyoD1.
Application
Anti-MyoD1 Antibody is a high quality Mouse Monoclonal Antibody for the detection of MyoD1 & has been validated in ICC, IHC, IP & WB.
Western blot: 1 μg/mL. Reacts with the ~45 kD protein.
Immunohistochemistry on frozen and formalin-fixed, paraffin embedded tissue: 2-4 μg/mL.
Immunocytochemistry
Immunoprecipitation: 2 μg/mg of protein lysate
Optimal working dilutions must be determined by end user.
Immunohistochemistry on frozen and formalin-fixed, paraffin embedded tissue: 2-4 μg/mL.
Immunocytochemistry
Immunoprecipitation: 2 μg/mg of protein lysate
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Recognizes MyoD1. The epitope is localized to amino acids 3-56 in the N-terminus of mouse MyoD1 protein.
Physical form
Format: Purified
Analysis Note
Control
Fetal Muscle, Rhabdomyosarcoma
Fetal Muscle, Rhabdomyosarcoma
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Related Content
A recurrent neomorphic mutation in MYOD1 defines a clinically aggressive subset of embryonal rhabdomyosarcoma associated with PI3K-AKT pathway mutations.
Kohsaka, S; Shukla, N; Ameur, N; Ito, T; Ng, CK; Wang, L; Lim, D; Marchetti, A; Viale et al.
Nature Genetics null
Francesca Megiorni et al.
Journal of hematology & oncology, 10(1), 161-161 (2017-10-08)
EPH (erythropoietin-producing hepatocellular) receptors are clinically relevant targets in several malignancies. This report describes the effects of GLPG1790, a new potent pan-EPH inhibitor, in human embryonal rhabdomyosarcoma (ERMS) cell lines. EPH-A2 and Ephrin-A1 mRNA expression was quantified by real-time PCR
Mark A Gillespie et al.
The Journal of cell biology, 187(7), 991-1005 (2009-12-23)
The mitogen-activated protein kinase p38-gamma is highly expressed in skeletal muscle and is associated with the dystrophin glycoprotein complex; however, its function remains unclear. After induced damage, muscle in mice lacking p38-gamma generated significantly fewer myofibers than wild-type muscle. Notably
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB3878 | 04053252725661 |