Anti-PPAR α Antibody

Peroxisome proliferator-activated receptors (PPARs) are nuclear hormone receptors that can be activated by a variety of compounds including fibratus, thiazolidinediones, prostaglandins and fatty acids. Three PPAR subtypes, designated PPARα, PPARβ (also designated PPARδ) and PPARγ, have been described. PPARs promote transcription by forming heterodimers with members of the retinoid X receptor (RXR) family of steroid receptors and binding to specific DNA motifs termed PPAR-response elements (PPREs). PPARα is abundant in primary hepatocytes where it regulates the expression of proteins involved in fatty acid metabolism. PPARβ is the most widely distributed subtype and is often expressed at high levels. PPARγ is predominantly seen in adipose tissue where it plays a critical role in regulating adipocyte differentiation.

Reacts with Peroxisome Proliferator Activated Receptor alpha (PPARalpha). No cross reactivity with PPARbeta or PPARgamma.

Synthetic peptide from amino acids 18-34 of mouse PPARalpha.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-PPAR α Antibody (Mouse Monoclonal Antibody) validated in ELISA, WB, ICC to detect PPAR alpha also known as Peroxisome Proliferator Activated Receptor α.

Western blot: 1:500-1:5,000

Immunocytochemistry: 1:500-1:5,000

ELISA: 1:500-1:5,000

Optimal working dilutions must be determined by end user.

Ascites fluid. Liquid. Contains no preservative.

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Control
3T3 whole cell lysate

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