MAB4120
Anti-MDR1 Antibody, clone JSB-1
culture supernatant, clone JSB-1, Chemicon®
Synonym(s):
P-glycoprotein, CD243, p170, Pgp
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-MDR1 Antibody, clone JSB-1, culture supernatant, clone JSB-1, Chemicon®
biological source
mouse
antibody form
culture supernatant
antibody product type
primary antibodies
clone
JSB-1, monoclonal
species reactivity
human, hamster
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
Quality Level
Gene Information
human ... ABCB1(5243)
Related Categories
Analysis Note
Control
MDR cells
MDR cells
Application
Anti-MDR1 Antibody, clone JSB-1 detects level of MDR1 & has been published & validated for use in FC, IC, IH(P) & WB.
Flow Cytometry
Immunohistochemistry on frozen and paraffin embedded tissue
sections: 1:20.
Immunocytochemistry: acetone or air-dried preparations react well.
Western blot
Note: For cellular detection, permeabilization is required.
Optimal working dilutions must be determined by end user.
Immunohistochemistry on frozen and paraffin embedded tissue
sections: 1:20.
Immunocytochemistry: acetone or air-dried preparations react well.
Western blot
Note: For cellular detection, permeabilization is required.
Optimal working dilutions must be determined by end user.
Research Category
Metabolism
Metabolism
Research Sub Category
Toxicology & Drug Resistance
Toxicology & Drug Resistance
Biochem/physiol Actions
Reacts with a conserved cytoplasmic epitope of the plasma membrane-associated 170 kDa P-gp, member of the superfamily of transmembrane transporters. JSB-1 detects P-gp overexpression in human tumor cells of all different histogenetic derivations.
JSB-1 does not cross-react with MDR3 P-glycoprotein. JSB-1 has been shown to cross-react with Pyruvate Carboxylase (PC), an abundant Mr 130,000 mitochondrial enzyme, on both immunoblots and immunohistochemical tissue sections [Rao et al. (1995). J Histo. Cytochem. 43(12):1187-1192.] Unequivocal plasma membrane patterns of immunostaining represent true P-glycoprotein ex-presssion. Weak homogeneous, cytoplasmic, or granular patterns of reactivity may represent staining of the PC cross-reactive epitope rather than positive staining for P-glycoprotein.
JSB-1 does not cross-react with MDR3 P-glycoprotein. JSB-1 has been shown to cross-react with Pyruvate Carboxylase (PC), an abundant Mr 130,000 mitochondrial enzyme, on both immunoblots and immunohistochemical tissue sections [Rao et al. (1995). J Histo. Cytochem. 43(12):1187-1192.] Unequivocal plasma membrane patterns of immunostaining represent true P-glycoprotein ex-presssion. Weak homogeneous, cytoplasmic, or granular patterns of reactivity may represent staining of the PC cross-reactive epitope rather than positive staining for P-glycoprotein.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
170 kDa
Immunogen
Epitope: Cytoplasmic
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
UnPurified mouse culture supernatant containing 0.7% BSA and 0.1% sodium azide as a preservative.
Unpurified
Preparation Note
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Andrea Ries Thurman et al.
Journal of acquired immune deficiency syndromes (1999), 78(1), 82-92 (2018-02-10)
We describe and compare the local and systemic pharmacokinetics (PK) of tenofovir (TFV) and TFV-diphosphate (TFV-DP) in healthy premenopausal (PRE) and postmenopausal (POST) women using TFV 1% gel and correlate local PK with other mucosal end points. PRE (n =
Matthew W Wilson et al.
Investigative ophthalmology & visual science, 47(4), 1269-1273 (2006-03-28)
To compare the expression of multidrug-resistant proteins in retinoblastoma tumors among eyes treated with primary enucleation. A group of 18 patients with unilateral retinoblastoma with advanced intraocular disease was selected for the study. All patients had undergone primary enucleation. A
Multidrug Resistance Gene (MDR-1) and Risk of Brain Metastasis in Epithelial Ovarian, Fallopian Tube, and Peritoneal Cancer.
Matsuo K, Eno ML, Ahn EH, Shahzad MM, Im DD, Rosenshein NB, Sood AK
American Journal of Clinical Oncology null
Daria Brambilla et al.
International journal of cancer, 130(12), 2824-2834 (2011-07-23)
Overexpression of the mdr1 gene encoding P-glycoprotein (Pgp) exerts a major role in reducing the effectiveness of cytotoxic therapy in osteosarcoma. The interaction between actin and Pgp has been shown to be instrumental in the establishment of multidrug resistance (MDR)
Multidrug resistance P-glycoprotein monoclonal antibody JSB-1 crossreacts with pyruvate carboxylase
Rao, V V, et al
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 43, 1187-1192 (1995)
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