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Merck
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MAB4349

Anti-TRA-2-49 Antibody, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-49/6E

clone TRA-2-49/6E, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
TRA-2-49/6E, monoclonal
Application:
FACS, ICC, IF, IP
Citations:
3

Key Documents

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biological source

mouse

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

TRA-2-49/6E, monoclonal

species reactivity

rabbit, feline, pig, human

should not react with

rat, monkey, guinea pig, mouse

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, immunoprecipitation (IP): suitable

input

sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

isotype

IgG1

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Related Categories

General description

212/80/55 kDa
In humans, four isoforms of alkaline phosphatase exist: intestinal, placental, placenta-like (germ cell type) and tissue-nonspecific. The tissue-nonspecific isoform of alkaline phosphatase is present in bone, liver and kidney; it differs from the other forms by posttranslational modification.
Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.

Immunogen

2102Ep human Embryonal Carcinoma Cells
Epitope: Liver/Bone/Kidney Alkaline Phosphatase

Application

Immunocytochemistry: A previous lot was used on IC.

Immunofluorescence: A previous lot of this antibody was used in IF.

FACS Analysis: A starting range of 5-20 µg/mL is suggested.

Immunoprecipitation: A previous lot of this antibody was used in IP.

Optimal working dilutions must be determined by end user.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
This Anti-TRA-2-49 Antibody, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-49/6E is validated for use in FC, IF, IC, IP for the detection of TRA-2-49.

Biochem/physiol Actions

Reactivity has been demonstrated with Liver/ Bone/Kidney isozyme of Alkaline Phosphatase (AP) from Humans, Higher Primates (Chimpanzee, Gorilla, Orangutan and Gibbon), New World Monkeys (Owl Monkey, Squirrel Monkey), Cat, Pig, Rabbit and Tiger. No reactivity has been observed with Old World Monkeys (Baboon, Rhesus monkey, Mangabey, Mandrill, Celebese Black Ape, African Green Monkey), Bovine, Canine, Goat, Guinea Pig, Hamster, Mouse, Rat and Sheep. This antibody does not cross react with other isozymes of AP.
Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used in flow cytometry to determine the expression of the Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence monitor the differentiation status of EC and ES cells.

Physical form

Protein A purified
Purified mouse monoclonal IgG1 liquid in buffer containing 0.02 M PBS, 0.25 M NaCl pH 7.6 with 0.1% Sodium Azide

Preparation Note

Stable for 1 year at from date of receipt.

Analysis Note

Control
Undifferentiated human embryonic stem cells (H9 line). NTERA-2 cl.D1 whole cell lysate (pluripotent stem cells derived from teratocarcinoma and are considered the malignant counterparts of human embryonic stem cells)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yu-Jing Liao et al.
Cell journal, 19(4), 640-646 (2017-11-07)
The importance of Oct4 and Sox2 in maintaining pluripotency and self-renewal is well-understood, but the functions of Klf4 and c-Myc has not been fully investigated. In the present study, we attempted to determine the roles of Klf4 and c-Myc on
Jenn-Fa Liou et al.
Scientific reports, 9(1), 15745-15745 (2019-11-02)
The objective of this study was to establish a versatile cell line for replication-incompetent virus production and inactivation with formaldehyde to generate a model of cell-based vaccine manufacturing process. To achieve this goal, we took advantage of the easily accessed

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