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UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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Product Name

Anti-Choline Acetyltransferase Antibody, clone 28C4, clone 28C4, Chemicon®, from mouse

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

28C4, monoclonal

species reactivity

human, rat, guinea pig, monkey

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_020549.3
NM_020984.2
NM_020985.2
NM_020986.2

UniProt accession no.

P28329

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CHAT(1103)
rat ... Chat(290567)
rhesus monkey ... Chat(709977)

Immunogen

Purified human placenta ChAT.

Application

Anti-Choline Acetyltransferase Antibody, clone 28C4 detects level of Choline Acetyltransferase & has been published & validated for use in ELISA, IH & WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers
Western blotting: 1:1,000-1:10,000 using a high sensitivity ECL kit. Recognizes a protein of ~67 kDa. May also recognize a protein of 35 kDa which is thought to be a degradation product. Suggested dilution buffer is TBS containing 5% skim milk powder and 0.05% Tween 20.

Immunohistochemistry on free-floating frozen sections of Rhesus monkey brain fixed with 4% paraformaldehyde and coronal vibratome sections of rat brain fixed with 4% paraformaldehyde and 0.2% glutaraldehyde.

ELISA (two side)

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Reacts with Choline Acetyltransferase (ChAT).

Physical form

Format: Purified
Purified immunoglobulin. Liquid.

Preparation Note

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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