Anti-NG2 Antibody, clone 132.39, Alexa Fluor^™488 Conjugate

NG2 is a high molecular weight, integral membrane chondroitin sulfate proteoglycan. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells (i.e., O-2A progenitor cells). NG2 is also found on the surfaces of chondroblasts, proliferating capillary endothelial cells, some human melanoma cell lines, and on leukemic blasts in childhood acute lymphoblastic leukemia. The NG2 proteoglycan is likely to play a role in regulation of cell motility, axon outgrowth and the cellular responses to platelet-derived growth factor.

The unconjugated parent antibody (Cat. No. MAB5384) has an observed molecular weight at ~280 kDa

Cell line expressing a truncated form of NG2

Anti-NG2 Antibody, clone 132.39, Alexa Fluor^™488 Conjugate detects level of NG2 & has been published & validated for use in ICC.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

This antibody recognizes the native, recombinant, and protein from cells which express NG2.

Protein G Purified

Purified mouse monoclonal IgG1 conjugated to Alexa Fluor^™ 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Control
Rat oligodendrocyte precursor cells

Evaluated by Immunocytochemistry in rat oligodendrocyte precursor cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NG2 in rat oligodendrocyte precursor cells. Performed without Triton.

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