MAB8419
Anti-Hepatitis B Virus Antibody, X-Protein, a.a. 90-115, clone 227
clone 227, Chemicon®, from mouse
Synonym(s):
Trans-Activator X Gene Product
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
227, monoclonal
Species reactivity:
human
Application:
ELISA, ICC, WB
Citations:
21
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
227, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunocytochemistry: suitable, western blot: suitable
isotype
IgG1κ
shipped in
wet ice
Quality Level
General description
Protein X (UniProt: P69713; also known as HBx, Peptide X, pX) is encoded by the X gene (Gene ID: 944566) in Hepatitis B virus. Hepatitis B virus (HBV) contains seven main proteins, which include Core, pre-Core, Small S, Middle S, Large S, Polymerase, and the HBx protein. HBx, a multifunctional protein, although not essential for HBV infection, plays a role in silencing host antiviral defenses and promoting viral transcription. It facilitates the efficient replication of HBV by stimulating HBV gene expression from the cccDNA template. It is mainly localized in the cytoplasm with a small fraction detected in the nucleus. However, its expression level can also influence its cellular localization. It is predominantly nuclear when expressed in cells at very low levels but becomes largely cytoplasmic as its expression level increases. In cytoplasm, a minor fraction may also associate with mitochondria. Its mitochondrial targeting sequence is localized in amino acids 68-117. HBx has an N-terminal negative regulatory domain and its transactivation or coactivation of the C-terminal domain is reported to interfere with host cell signaling transduction pathways to help HBV replication. The best-characterized HBx binding partner is the cellular damage-specific DNA binding protein 1 (DDB1) and the interaction of HBx-DDB1 is essential for HBV replication. HBx promotes the degradation of the cellular structural maintenance of chromosomes 5/6 complex (Smc5/6), which directly binds DNA and entraps DNA plasmids. HBx is directly involved in development of cirrhosis and liver and hepatocellular carcinoma. (Ref.: Kornyeyev, D., et al. (2019). J. Virol. 93(16); e00248-19; Hwang, G-Y., et al. (2003). J. Clin. Microbiol. 41(12); 5598-5603).
Immunogen
Epitope: X-Protein, aa 90-115
Peptide cooresponding to aa90-115 of h HBV-X protein.
Application
Anti-Hepatitis B Virus Antibody, X-Protein, a.a. 90-115, clone 227 detects level of Hepatitis B Virus & has been published & validated for use in ELISA, WB & IC.
ELISA, immunoblotting and immunocytochemistry staining of infected liver tissues and HBV-transfected hepatoma cells. Optimal working dilutions must be determined by the end user.
Research Category
Infectious Diseases
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral
Infectious Diseases - Viral
Biochem/physiol Actions
Reacts with Hbx-protein (trans-activator X gene product) of hepatitis B virus (epitope recognized - amino acid positions 90-115).
SPECIES REACTIVITIES:
Reacts only with HBV-infected human cells.
SPECIES REACTIVITIES:
Reacts only with HBV-infected human cells.
Physical form
Format: Purified
Liquid in 0.02M PBS pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to 12 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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HBX causes cyclin D1 overexpression and development of breast cancer in transgenic animals that are heterozygous for p53.
Klein, A; Guhl, E; Tzeng, YJ; Fuhrhop, J; Levrero, M; Graessmann, M; Graessmann, A
Oncogene null
Accumulation of 8-hydroxy-2'-deoxyguanosine adducts in HBx recombinant HepG2 cells and HBx transgenic mice.
Ralph Gehrke, Maria A Brauchle, Kurt Reifenberg, Eberhard Hildt, Uwe Gruetzner et al.
Digestion null
Ha-Yeon Lim et al.
Viruses, 14(10) (2022-10-28)
HBx, a multifunctional regulatory protein, plays an essential role in the replication and pathogenesis of the hepatitis B virus (HBV). In this study, we found that in human hepatoma cells, the tumor suppressor p53 downregulates HBx via ubiquitin-dependent proteasomal degradation.
Epigenetic modification induced by hepatitis B virus X protein via interaction with de novo DNA methyltransferase DNMT3A.
Da-Li Zheng, Li Zhang, Na Cheng, Xiao Xu, Qing Deng, Xiao-Mei Teng, Ke-Sheng Wang et al.
Journal of Hepatology null
Yongdong Niu et al.
Journal of clinical and translational hepatology, 9(4), 466-476 (2021-08-28)
Hepatitis B virus (HBV) infection has been found to increase hepatocellular sensitivity to carcinogenic xenobiotics, by unknown mechanisms, in the generation of hepatocellular carcinoma. The pregnane X receptor (PXR) is a key regulator of the body's defense against xenobiotics, including
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