MAB8566
Anti-Poliovirus Blend Antibody, clones 583-G8-G2-A4, 591-B1-H7-D1, and 613-F1-B5-E5
ascites fluid, Chemicon®, from mouse
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
583-G8-G2-A4, monoclonal, 591-B1-H7-D1, monoclonal, 613-F1-B5-E5, monoclonal
Species reactivity:
human
Application:
IF
Citations:
2
biological source
mouse
antibody form
ascites fluid
antibody product type
primary antibodies
clone
583-G8-G2-A4, monoclonal, 591-B1-H7-D1, monoclonal, 613-F1-B5-E5, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
immunofluorescence: suitable
isotype
IgG
shipped in
dry ice
Quality Level
Application
Detect Poliovirus Blend using this Anti-Poliovirus Blend Antibody, clones 583-G8-G2-A4, 591-B1-H7-D1 & 613-F1-B5-E5 validated for use in IF.
IFA at 1:100-1:400.
Dilute with buffer pH 7.5-8.0 to desired working volume.
Final working dilutions must be determined by end user.
SUGGESTED IFA PROTOCOL:
Substrate slides:
1. Scrape and spot cells onto microscope slide wells.
2. Air dry.
3. Fix in cold acetone for 10 minutes at +4°C.
Stain:
1. Add 15 μl diluted polio antibody.
2. Incubate at +37°C for 30 minutes in a humid chamber.
3. Rinse in PBS for 5 minutes; repeat with fresh PBS.
4. Add 15 μl diluted labeled antibody.
5. Repeat steps 2 and 3.
6. Mount with buffered glycerol.
Dilute with buffer pH 7.5-8.0 to desired working volume.
Final working dilutions must be determined by end user.
SUGGESTED IFA PROTOCOL:
Substrate slides:
1. Scrape and spot cells onto microscope slide wells.
2. Air dry.
3. Fix in cold acetone for 10 minutes at +4°C.
Stain:
1. Add 15 μl diluted polio antibody.
2. Incubate at +37°C for 30 minutes in a humid chamber.
3. Rinse in PBS for 5 minutes; repeat with fresh PBS.
4. Add 15 μl diluted labeled antibody.
5. Repeat steps 2 and 3.
6. Mount with buffered glycerol.
Biochem/physiol Actions
Reacts with the Poliovirus 1, 2 and 3.
Preparation Note
Maintain at -20°C in convenient undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Johanna Marsian et al.
Nature communications, 8(1), 245-245 (2017-08-16)
Poliovirus (PV) is the causative agent of poliomyelitis, a crippling human disease known since antiquity. PV occurs in two distinct antigenic forms, D and C, of which only the D form elicits a robust neutralizing response. Developing a synthetically produced
Mohammad W Bahar et al.
NPJ vaccines, 6(1), 5-5 (2021-01-10)
Global vaccination programs using live-attenuated oral and inactivated polio vaccine (OPV and IPV) have almost eradicated poliovirus (PV) but these vaccines or their production pose significant risk in a polio-free world. Recombinant PV virus-like particles (VLPs), lacking the viral genome
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