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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

DCS-35, monoclonal

species reactivity

mouse, pig, rat, human

manufacturer/tradename

Chemicon®

technique(s)

activity assay: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NM_000075.2

UniProt accession no.

P11802

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CDK4(1019)
rat ... Cdk4(94201)

General description

The prototype of the cdk family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression.

Specificity

Recognizes a 34 kDa protein identified as p34 or cyclin-dependent kinase 4 (cdk4). It shows no cross-reaction with other cdks.

Immunogen

Raised against purified recombinant cdk4 protein.

Application

Detect Cdk4 with Anti-Cdk4 Antibody, clone DCS-35 (Mouse Monoclonal Antibody), that has been shown to work in IF, IP, EA, WB & IHC.
Immunofluorescence

Immunoprecipitation: excellent for coprecipitation of D-type cyclins bound to cdk4.

Western Blotting: 2-4 ug/mL

Kinase Assay

The prototype of the cdk family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression.

Optimal working dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair

Linkage

Replaces: MABE548

Physical form

Format: Purified
Purified immunoglobulin. Liquid containing 10 mM PBS, pH 7.4 with 0.2% BSA and 0.09% sodium azide.

Storage and Stability

Maintain liquid material at 2-8°C for up to 24 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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