Anti-Stat3b Antibody, clone 516 G10H9

83.13 kDa calculated. Uncharacterized band(s) may appear in some lysates.

Signal transducer and activator of transcription 3 (UniProt P40763; also known as Acute-phase response factor, DNA-binding protein APRF) is encoded by the STAT3 (also known as ADMIO, APRF, HIES) gene (Gene ID 6774) in human. STAT proteins (1, 2, 3, 4, 5A, 5B, 6) constitute a family of proteins that mediate cytokine and growth factor receptors-initiated signaling events. Stat3 is activated upon phosphorylation on Y705 by Jak or receptor tyrosine kinases, leading to Stat3 homodimerization, nuclear translocation, and target genes transcription. Constitutively tyrosine-phosphorylated Stat3 is implicated in the initiation and progression of many types of cancers, while Stat3 gene knockout in mice is lethal at the early embryonic stage. Two major alternatively spliced Stat3 isoforms exist, the C-terminal end 55-amino acid sequence (a.a. 716-770) in the canonical alpha isoform (UniProt P40763-1) is truncated and replaced with a 7-amino acid sequence in the 722-amino acid beta isoform (UniProt P40763-3). Tyrosine-phosphorylated Stat3β homodimers are more stable and less susceptible to dephosphorylation, bind DNA more avidly and exhibit markedly prolonged nuclear retention than pStat3α homodimers.

Epitope: Within Stat3beta C-terminal end 7 a.a. (CT7).

Linear peptide containing human Stat3b C-terminal end 7 a.a. (CT7).

Anti-Stat3b Antibody, clone 516 G10H9 is an antibody against Stat3b for use in Western Blotting, Immunoprecipitation.

Research Category
Apoptosis & Cancer

Research Sub Category
Transcription Factors

Western Blotting Analysis: 1 µg/mL from a representative lot detected 0.3-100 ng of human Stat3β (a.a. 127–722), but not Stat3α (a.a. 127–770), GFP fusion protein (Courtesy of Dr. David J. Tweardy, Baylor College of Medicine, Houston, TX).
Western Blotting Analysis: A representative lot specifically detected Stat3β (a.a. 127–722) GFP fusion protein, but not Stat3α (a.a. 127–770) or Stat3βΔCT7 (a.a. 127–717) GFP fusion that lacks Stat3β C-terminal end 7-a.a. (CT7) sequence (Bharadwaj, U., et al. (2014). Cancers (Basel). 6(4):2012-2034).
Immunoprecipitation Analysis: A representative lot specifically immunoprecipitated human Stat3β (a.a. 127–722) GFP fusion protein, but not Stat3α (a.a. 127–770) GFP fusion protein, exogenously expressed in MEFs lacking endogenous murine Stat3 (Bharadwaj, U., et al. (2014). Cancers (Basel). 6(4):2012-2034).

Clone 516 G10H9 detects the C-terminal end 7-a.a. (CT7) sequence present in human/mouse spliced isoform Stat3B (UniProt P40763-3/P42227-2), but not in human/mouse spliced isoform Stat3A (UniProt P40763-1/P42227-1) or human/mouse isoform Del-701 (UniProt P40763-2/P42227-3).

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evalusated by Western Blotting of recombinant human Stat3β GFP fusion protein.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected 0.5 µg of human Stat3β (a.a. 127–722) GFP fusion protein.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.