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MABC186-I

Anti-phospho-p62 (Ser403) Antibody, clone 4F6

clone 4F6, from rat

Synonym(s):

Ubiquitin-binding protein p62, p62, EBI3-associated protein of 60 kDa, EBIAP, p60, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43
eCl@ss:
32160702

Key Documents

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Product Name

Anti-phospho-p62 (Ser403) Antibody, clone 4F6, clone 4F6, from rat

biological source

rat

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4F6, monoclonal

species reactivity

human, mouse

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_003891.1

UniProt accession no.

Q13501

shipped in

ambient

target post-translational modification

phosphorylation (pSer403)

Quality Level

100

Gene Information

human ... SQSTM1(8878)

Analysis Note

Evaluated by Western Blotting in Neuro2a cells.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected p62/SQSTM1 (Ser403) in Neuro2a cells in which GFP fused human p62 (G-p62) was stably transfected and treated with MG132 vs. an untreated sample.

Application

Detect p62/SQSTM1 (Ser403) using this rat monoclonal Anti-phospho-p62 (Ser403) antibody, clone 4F6, Cat. No. MABC186-I. Validated for use in Immunohistochemistry and Western Blotting.
Western Blotting Analysis: A representative lot detected p62/SQSTM1 (Ser403) in a Western Blot application. (Matsumoto, G., et. al. (2011). Mol Cell. 44(2):279-289).

Immunohistochemistry Analysis: A representative lot detected p62/SQSTM1 (Ser403) in a Immunohistochemistry application. (Matsumoto, G., et. al. (2011). Mol Cell. 44(2):279-289).





Biochem/physiol Actions

Clone 4F6 recognizes p62/SQSTM1 phosphorylated at Ser403 in human and mouse.

General description

Sequestosome-1 (UniProt: Q13501; also known as EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62) is encoded by the SQSTM1 (also known as ORCA, OSIL) gene (Gene ID 8878) in human Sequestosome is a multi-functional co-interacting protein with a UBA domain at its C-terminal end, which binds non-covalently to polyubiquitin chains and regulates the activation of the nuclear factor kappa-B (NF-kappaB) signaling. It functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 (TRAF6) to mediate the activation of NF- B in response to upstream signals. It also serves as an autophagosome cargo protein that targets other proteins that bind to it for selective autophagy. Defects in sequestosome-1 are a cause of Paget disease of bone (PDB), a metabolic bone disease affecting the axial skeleton and is characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Mutations in SQSTM1 gene can lead to frontotemporal dementia and/or amyotrophic lateral sclerosis 3. Frontotemporal dementia is characterized by frontal and temporal lobe atrophy associated with neuronal loss, gliosis, and dementia. Amyotrophic lateral sclerosis is characterized by the death of motor neurons in the brain, brainstem, and spinal cord, resulting in fatal paralysis.
~60 kDa observed; 46.68 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Immunogen

KLH-conjugated linear peptide corresponding to a sequence surrounding pSer403 of human phospho p62/SQSTM1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

Certificates of Analysis (COA)

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Valeria Gerbino et al.
Neuron, 106(5), 789-805 (2020-03-30)
DNA sequence variants in the TBK1 gene associate with or cause sporadic or familial amyotrophic lateral sclerosis (ALS). Here we show that mice bearing human ALS-associated TBK1 missense loss-of-function mutations, or mice in which the Tbk1 gene is selectively deleted
Zhiqiang Deng et al.
Autophagy, 16(5), 917-931 (2019-08-01)
Macroautophagy (autophagy) is a key catabolic pathway for the maintenance of proteostasis through constant digestion of selective cargoes. The selectivity of autophagy is mediated by autophagy receptors that recognize and recruit cargoes to autophagosomes. SQSTM1/p62 is a prototype autophagy receptor

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