Skip to Content
Merck
CN

MABC605

Anti-TXNIP Antibody, clone 3A7.1

clone 3A7.1, from mouse

Synonym(s):

Thioredoxin-interacting protein, Thioredoxin-binding protein 2, Vitamin D3 up-regulated protein 1

Sign In to View Organizational & Contract Pricing

Select a Size

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

3A7.1, monoclonal

species reactivity

mouse, human

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NP_006463

UniProt accession no.

Q9H3M7

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TXNIP(10628)

General description

Thioredoxin-interacting protein (UniProt Q9H3M7; also known as Thioredoxin-binding protein 2, Vitamin D3 up-regulated protein 1) is encoded by the TXNIP (also known as VDUP1) gene (Gene ID 10628) in human. Thioredoxin interacting protein (TXNIP) binds thioredoxin active cysteine residue and suppresses its antioxidant activity. TXNIP overexpression results in increased cellular ROS level and oxidative cell death, while TXNIP suppression enhances cellular resistance to oxidative injury. TXNIP is also shown to inhibit cellular glucose uptake by downregulating glucose transporter 1 (GLUT1) plasma membrane localization, and TXNIP expression is found to be markedly suppressed in many cancers, making TXNIP a potential anticancer therapeutic target.
~44 kDa observed. 43.66/37.32 kDa (human isoform 1/2) and 44.36/44.29 kDa (mouse isoform 1/2) calculated.

Immunogen

GST-tagged recombinant fragment from the N-terminal half of human TXNIP.

Application

Anti-TXNIP Antibody, clone 3A7.1 is an antibody against TXNIP for use in Western Blotting, Immunocytochemistry.
Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected TXNIP in HUVECs, A431, HeLa, and NIH/3T3 cells.

Biochem/physiol Actions

Clone 3A7.1 targets an epitope within the N-terminal half of TXNIP. Cross-reactivity to human and mouse spliced isoform 2 is likely, but has not been confirmed.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected TXNIP in 10 µg of K562 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service