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MABD122C3

Anti-CD73 Antibody, clone AA60-E3-3, Cy3 Conjugate

clone AA60-E3-3, from mouse, CY3 conjugate

Synonym(s):

CD-73, 5′-nucleotidase, 5′-NT, ecto-5′-nucleotidase

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.44

Key Documents

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biological source

mouse

Quality Level

100

conjugate

CY3 conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

AA60-E3-3, monoclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (immunogen homology)

technique(s)

immunocytochemistry: suitable

isotype

IgG1κ

NCBI accession no.

NP_001191742

UniProt accession no.

Q61503

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NT5E(4907)

General description

CD73, also known as Ecto-5′-nucleotidase, catalyzes the conversion of purine 5′ mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation, being expressed on subsets of B-cells and T-cells. CD73 is also a marker of undifferentiated mesenchymal stem cells and is often used to identified stem cell populations.
The unconjugated parent antibody (Catalog No. MABD122) has an observed MW at 63 kDa

Immunogen

Whole Mouse Dermal Lymphatic Endothelial Cells (DLEC)

Application

Research Category
Stem Cell Research
Research Sub Category
Mesenchymal Stem Cells
This Anti-CD73 Antibody, clone AA60-E3-3, Cy3 Conjugate is validated for use in Immunocytochemistry for the detection of CD73.

Physical form

Protein G Purified
Purified mouse monoclonal IgG1 conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.

Preparation Note

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Evaluated by Immunocytochemistry on human adipose and bone marrow mesenchymal stem cells.
Immunocytochemical analysis: A 1:100 dilutions of this antibody detected CD73 on 1X10^6 human adipose and bone marrow mesenchymal stem cells.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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