MABE1023
Anti-Mitotic Checkpoint Protein BUB3 Antibody, clone 1A3.1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
1A3.1, monoclonal
Application:
ICC, WB
Citations:
-
conjugate
unconjugated
antibody form
culture supernatant
clone
1A3.1, monoclonal
purified by
(unpurified)
concentration
(Please refer to lot specific datasheet.)
technique(s)
immunocytochemistry: suitable, western blot: suitable
isotype
IgG2bκ
UniProt accession no.
target post-translational modification
unmodified
Quality Level
species reactivity
human
General description
GST-tagged recombinant fragment corresponding to the WD repeats 6 & 7 of human BUB3.
~37 kDa observed. 37.16 kDa (isoform 1) and 36.96 kDa (isoform 2) calculated.
Application
Epitope: WD repeats 6 & 7.
Biochem/physiol Actions
Expected to react with both spliced isoforms reported by UniProt (O43684).
Physical form
Mouse monoclonal IgG2bκ antibody in supernatant without preservatives.
Preparation Note
Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in C2C12 mouse myoblast lysate.
Western Blotting Analysis: A 1:250 dilution of this antibody detected Mitotic Checkpoint Protein BUB3 in 10 µg of C2C12 mouse myoblast lysate.
Western Blotting Analysis: A 1:250 dilution of this antibody detected Mitotic Checkpoint Protein BUB3 in 10 µg of C2C12 mouse myoblast lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
10 - Combustible liquids
Regulatory Information
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