Anti-Mitotic Checkpoint Protein BUB3 Antibody, clone 1A3.1

GST-tagged recombinant fragment corresponding to the WD repeats 6 & 7 of human BUB3.

~37 kDa observed. 37.16 kDa (isoform 1) and 36.96 kDa (isoform 2) calculated.

Epitope: WD repeats 6 & 7.

Expected to react with both spliced isoforms reported by UniProt (O43684).

Mouse monoclonal IgG2bκ antibody in supernatant without preservatives.

Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Western Blotting in C2C12 mouse myoblast lysate.

Western Blotting Analysis: A 1:250 dilution of this antibody detected Mitotic Checkpoint Protein BUB3 in 10 µg of C2C12 mouse myoblast lysate.

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