MABE126
Anti-SR protein family Antibody, clone 16H3
clone 16H3, from mouse
Synonym(s):
splicing factor, arginine/serine-rich 6, arginine/serine-rich splicing factor 6, splicing factor, arginine/serine-rich, 55 kDa, Pre-mRNA-splicing factor SRP55, pre-mRNA splicing factor SRP55
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
16H3, monoclonal
Application:
ICC, IP, WB
Citations:
5
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
16H3, monoclonal
species reactivity
human
species reactivity (predicted by homology)
mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SRSF6(6431)
General description
Multiple SR protein family members detected. Reported to predominantly detect SRp75, SRp55, SRp40, SRp30, SRp20 (Neugebauer, KM, et al. (1995).
Ser-Arg-rich (SR) proteins make up a family of functionally and structurally conserved phosphoproteins, and are required components of alternative and constitutive pre-mRNA splicing. SR proteins are characterized by their modular composition consisting of two domains of interest: an N-terminal RNA recognition motif (RRM) which serves in the determination of DNA-binding specificity, and the RS domain, an arginine and serine rich, C-terminal domain that serves as a shuttling and localization director of SR proteins and as a splicing activation domain. They are involved in various aspects of pre-mRNA splicing and in spliceosome assembly including the identification and appropriation of splice-sites and the manipulation of alternative splicing regulation.
Immunogen
Dephosphorylated bovine SRp55 and GST-tagged recombinant dephosphorylated SRp55 (Neugebauer, KM et. al 1995).
Application
Anti-SR protein family Antibody, clone 16H3 is a Mouse Monoclonal Antibody for detection of SR protein family also known as arginine/serine-rich splicing factor 6 & has been validated in WB, IP, ICC.
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP (Neugebauer, KM, et al. (1995).
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. (Neugebauer, KM, et al. (1995).
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. (Neugebauer, KM, et al. (1995).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins
RNA Metabolism & Binding Proteins
Biochem/physiol Actions
This antibody recognizes the four nuclear proteins SRp75, SRp55, SRp40, and SRp20, plus ~20 other prominent nuclear proteins believed to correspond to the SR family.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa nuclear extract
HeLa nuclear extract
Evaluated by Western Blot in HeLa nuclear extract.
Western Blot Analysis: 0.5 µg/mL of this antibody detected SR proteins in 10 µg of HeLa nuclear extract.
Western Blot Analysis: 0.5 µg/mL of this antibody detected SR proteins in 10 µg of HeLa nuclear extract.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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María Luz Morales et al.
Leukemia, 37(8), 1649-1659 (2023-07-09)
Despite the approval of several drugs for AML, cytarabine is still widely used as a therapeutic approach. However, 85% of patients show resistance and only 10% overcome the disease. Using RNA-seq and phosphoproteomics, we show that RNA splicing and serine-arginine-rich
Komal Raina et al.
Nucleus (Austin, Tex.), 13(1), 58-73 (2022-03-01)
Nuclear Speckles (NS) are phase-separated condensates of protein and RNA whose components dynamically coordinate RNA transcription, splicing, transport and DNA repair. NS, probed largely by imaging studies, remained historically well known as Interchromatin Granule Clusters, and biochemical properties, especially their
Morgane Corre et al.
Nucleic acids research, 51(22), 12459-12475 (2023-11-09)
Cell autonomous responses to intracellular bacteria largely depend on reorganization of gene expression. To gain isoform-level resolution of these modes of regulation, we combined long- and short-read transcriptomic analyses of the response of intestinal epithelial cells to infection by the
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MABE126 | 04053252553363 |