MABE1821
Anti-phospho-HDAC1 (Ser406) Antibody, clone BT-15
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
conjugate
unconjugated
antibody form
purified antibody
clone
BT-15
monoclonal
purified by
using protein G
species reactivity
mouse, human
concentration
(Please refer to lot specific datasheet.)
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2bκ
UniProt accession no.
target post-translational modification
phosphorylation (pSer406)
General description
60 kDa observed. Uncharacterized bands may be observed in some lysate(s).
KLH-conjugated linear peptide corresponding to 19 amino acids surrounding phosphoserine 406 from the C-terminal region of human Histone deacetylase 1.
Application
Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho-HDAC1 (Ser406) in Immunoprecipitation applications (Segre, C.V., et. al. (2016). MAbs. 8(1):37-42).
Immunocytochemistry Analysis: A representative lot detected phospho-HDAC1 (Ser406) in Immunocytochemistry applications (Segre, C.V., et. al. (2016). MAbs. 8(1):37-42).
Immunocytochemistry Analysis: A representative lot detected phospho-HDAC1 (Ser406) in Immunocytochemistry applications (Segre, C.V., et. al. (2016). MAbs. 8(1):37-42).
Biochem/physiol Actions
Clone BT-15 is a mouse monoclonal antibody that specifically detects Histone deacetylase phosphorylated on serine 406 in mitotic synchronized cells. It targets an epitope within 19 amino acids from the C-terminal region.
Physical form
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in lysate from HeLa cells pretreated with anti-mitotic agent (Nocodazole).
Western Blotting Analysis: 2 µg/mL of this antibody detected phospho-HDAC1 (Ser406) in lysates from HeLa cells pretreated with Nocodazole (330 nM for 16 h).
Western Blotting Analysis: 2 µg/mL of this antibody detected phospho-HDAC1 (Ser406) in lysates from HeLa cells pretreated with Nocodazole (330 nM for 16 h).
Other Notes
Human. Predicted to react with Bovine, Monkey, Rat, Rabbit based on 100% sequence homology.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Regulatory Information
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