Anti-phospho-HDAC1 (Ser406) Antibody, clone BT-15

60 kDa observed. Uncharacterized bands may be observed in some lysate(s).

KLH-conjugated linear peptide corresponding to 19 amino acids surrounding phosphoserine 406 from the C-terminal region of human Histone deacetylase 1.

Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho-HDAC1 (Ser406) in Immunoprecipitation applications (Segre, C.V., et. al. (2016). MAbs. 8(1):37-42).

Immunocytochemistry Analysis: A representative lot detected phospho-HDAC1 (Ser406) in Immunocytochemistry applications (Segre, C.V., et. al. (2016). MAbs. 8(1):37-42).

Clone BT-15 is a mouse monoclonal antibody that specifically detects Histone deacetylase phosphorylated on serine 406 in mitotic synchronized cells. It targets an epitope within 19 amino acids from the C-terminal region.

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in lysate from HeLa cells pretreated with anti-mitotic agent (Nocodazole).

Western Blotting Analysis: 2 µg/mL of this antibody detected phospho-HDAC1 (Ser406) in lysates from HeLa cells pretreated with Nocodazole (330 nM for 16 h).

Human. Predicted to react with Bovine, Monkey, Rat, Rabbit based on 100% sequence homology.

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