MABE326
Anti-JARID1D/KDM5D Antibody, clone 7F10.4
clone 7F10.4, from mouse
Synonym(s):
JARID1D/KDM5D, Lysine-specific demethylase 5D, Histocompatibility Y antigen, H-Y, Histone demethylase JARID1D, Jumonji/ARID domain-containing protein 1D, Protein SmcY
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
7F10.4, monoclonal
Application:
ICC, WB
Citations:
-
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
7F10.4, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... KDM5D(8284)
General description
Lysine-specific demethylase 5D (UniProt Q9BY66; also known as H-Y, Histone demethylase JARID1D, Histocompatibility Y antigen, Human homolog of selected mouse cDNA on Y, Jumonji/ARID domain-containing protein 1D, Jumonji AT rich interactive domain 1D (RBP2-like), Protein SmcY, SMC homolog Y chromosome, Smcy homolog Y-linked) is encoded by the KDM5D (also known as HY, HYA, JARID1D, KIAA0234, SMCY) gene (Gene ID 8284) in human. JARID1D/KDM5D is a histone demethylase that specifically catalyzes the demethylation of tri- and di-methylated Lys4 of histone H3 (H3K4m3 and H3K4m2), but not methylated H3K9, H3K27, H3K36, H3K79, H4K20, or monomethylated H3K4. The KDM5D gene is mapped to the azoospermia factor (AZF) region known to be involved in spermatogenesis.
~200 kDa observed. Isoform 1 (174.1 kDa calculated); Isoform 2 (167.4 kDa calculated); Isoform 3 (177.6 kDa calculated). Uncharacterized band(s) may appear in some lysates.
Immunogen
KLH-conjugated linear peptide corresponding to human JARID1D/KDM5D.
Application
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected JARID1D/KDM5D in HeLa cells.
This Anti-JARID1D Antibody, clone 7F10.4 is validated for use in Western Blotting and Immunocytochemistry for the detection of KDM5D .
Biochem/physiol Actions
Clone 7F10.4 is expected to react with all three spliced JARID1D/KDM5D isoforms.
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blotting in U2OS cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected JARID1D/KDM5D in 10 µg of U2OS cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected JARID1D/KDM5D in 10 µg of U2OS cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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