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MABE913

Anti-Ikaros Antibody, clone Ik14

clone Ik14, from mouse

Synonym(s):

DNA-binding protein Ikaros, Ikaros family zinc finger protein 1, Lymphoid transcription factor LyF-1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
Ik14, monoclonal
Application:
ChIP
western blot
Species reactivity:
human
Citations:
Technique(s):
ChIP: suitable (ChIP-seq)
western blot: suitable
Uniprot accession no.:
Q13422

Key Documents

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Product Name

Anti-Ikaros Antibody, clone Ik14, clone Ik14, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

Ik14, monoclonal

species reactivity

human

technique(s)

ChIP: suitable (ChIP-seq)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_001207694

UniProt accession no.

Q13422

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... IKZF1(10320)

Analysis Note

Evaluated by Western Blotting in a Jurkat cell lysate.

Western Blotting Analysis: A 0.5 µg/mL dilution of this antibody detected Ikaros in 10 µg of Jurkat cell lysate.

Application

Anti-Ikaros, clone Ik14 is an antibody against Ikaros for use in WB, ChIP, ChIP-seq.
Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Chromatin Biology
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Ikaros in 10 µg of HL-60 and human lymph node lysate.
Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody as part of a blend Ikaros clones Ik14 and E9 (catalogue numbers MABE913 and MABE912 respectively) was demonstrated to perform in ChIP. See Zhang et al. (2012).
ChIP-seq Analsysis: A representative lot of this antibody as part of a blend Ikaros clones E9 and Ik14 (catalogue number MABE912 and MABE913 respectively) was demonstrated to perform in ChIP-seq. See Zhang et al. (2012).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Ikaros, also known as IKZF1, is a transcription factor that is important for the regulation of lymphopoiesis and also the development of the immune system. It has also been linked to adult and fetal erythroid differentiation in humans. Ikaros has been shown to induce local chromatin accessibility during CD4+ T cell differentiation and therefore, activating Th2 expression.
~60 kDa observed

Immunogen

Recombinant protein corresponding to Human Ikaros.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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