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MABE915

Anti-Mi-2β Antibody, clone 2G8

clone 2G8, from mouse

Synonym(s):

Chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, Mi-2b

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
2G8, monoclonal
Conjugate:
unconjugated
Application:
ChIP
western blot
Species reactivity:
human
Citations:
Technique(s):
ChIP: suitable (ChIP-seq)
western blot: suitable
Uniprot accession no.:
Q14839

Key Documents

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Product Name

Anti-Mi-2β Antibody, clone 2G8, clone 2G8, from mouse

clone

2G8, monoclonal

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

species reactivity

human

technique(s)

ChIP: suitable (ChIP-seq)
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NP_001264

UniProt accession no.

Q14839

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... CHD4(1108)

Analysis Note

Evaluated by Western Blotting in a Jurkat cell lysate.

Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Mi-2β in 10 µg of a Jurkat cell lysate.

Application

Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody used as part of an antibody cocktail of consisting of Anti-Mi-2β clones 2G8, 16G4 and 17H11 (cat. no. MABE915, MABE914, and MABE920 respectively) was reported to work in ChIP (see Zhang, J., et al 2012).

ChIP-seq: A representative lot of this antibody used as part of an antibody cocktail of consisting of Anti-Mi-2β clones 2G8, 16G4 and 17H11 (cat. no. MABE915, MABE914, and MABE920 respectively) was reported to work in ChIP-seq (see Zhang, J., et al 2012).
This Anti-Mi-2β Antibody, clone 2G8 is validated for use in Western Blotting and ChIP-seq and Culture Confirmation for the detection of Mi-2β.

General description

Chromodomain-helicase-DNA-binding protein 4 (CHD-4), often referred to as Mi-2β, is a central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. Mi-2β has been shown to interact with TRIM27, HDAC1, Histone deacetylase 2, MTA2, SATB1 and Ataxia telangiectasia and Rad3 related. This protein also interacts with KLF1. Sumoylation of KLF1 leads to transcriptional repression of Mi-2β.
~ 220 kDa observed

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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