Anti-IDO-2 Antibody, clone 4-3

Indoleamine 2,3-dioxygenase 2 (UniProt: Q8R0V5; also known as IDO-2, Indoleamine 2,3-dioxygenase-like protein 1, Indoleamine-pyrrole 2,3-dioxygenase-like protein 1) is encoded by the Ido2 (also known as Indol1) gene (Gene ID: 209176) in murine species. IDO-2 is expressed mainly in antigen-presenting immune cells, liver, kidney, brain, and placenta. It is highly expressed in kidney, followed by epididymis and liver and is also detected in the tails of the spermatozoa. It catalyzes the first and rate-limiting step in the kynurenine pathway of tryptophan catabolism. IDO-1 and IDO-2 are 2 distinct enzymes which catalyze the same reaction. However, the Km for IDO-2 for tryptophan is much higher than that of IDO-1. IDO-2 may play a role as a negative regulator of IDO-1 by competing for heme-binding with IDO-1. IDO-2 activity is inhibited by 1MT (1-methyl-tryptophan) and MTH-trp (methylthiohydantoin-DL-tryptophan). IDO-2 is also involved in immune regulation. IDO-2 knockout mice do not show any apparent defects in their embryonic development or hematopoietic differentiation and have wild-type profiles for kynurenine in blood serum and for immune cells in spleen, lymph nodes, peritoneum, thymus and bone marrow. However, the knockout mice exhibit defects in IDO-mediated T-cell regulation and inflammatory responses. (Ref.: Mbongue JC, et al. (2015). Vaccines (Basel). 3(3):703-29).

~55 kDa observed; 45.25 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

KLH-conjugated linear peptide/recombinant protein corresponding to 21 amino acids from the C-terminal region of murine Indoleamine 2,3-dioxygenase 2 (IDO-2).

Anti-IDO-2, clone 4-3, Cat. No. MABF2055, is a mouse monoclonal antibody that detects IDO2 and has been tested for use in Western Blotting.

Research Category
Inflammation & Immunology

Western Blotting Analysis: 0.5 µg/mL from a represesntative lot detected IDO-2 in 10 µg of mouse kidney tissue lysate.

Clone 4-3 detects IDO-2 in mouse. It targets an epitope within 21 amino acids in the C-terminal region.

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in mouse liver tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected IDO-2 in 10 µg of mouse liver tissue lysate.

Concentration: Please refer to lot specific datasheet.

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