36.13 kDa calculated.

Natural cytotoxicity triggering receptor 1 (UniProt: S4S7F8; also known as Lymphocyte antigen 94 homolog, NK cell-activating receptor, Natural killer cell p46-related protein, NK-p46, NKp46, CD335) is encoded by the NCR1 gene (Gene ID: 611390) in canine species. NK-p46 is a single-pass type I membrane protein that belongs to the natural cytotoxicity receptor (NCR) family and serves as a cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis and elimination of virus-infected cells. It is considered as a primary activating receptor mediating NK cell antiviral function. It is involved in the functional recognition of viral HA and hemagglutinin-neuraminidases. Following the initiation of an NK-target cell interaction, NKp46 is reported to cluster at the cell membrane, specifically at the immune synapse where it mediates cytoskeletal rearrangement and cellular polarization. Canine NK-p46 is synthesized with a signal peptide of 20 amino acids, which is subsequently cleaved off. The mature form has an extracellular domain (aa 21-255), a helical domain (aa 256-279), and a cytoplasmic domain (aa 280-316). It also has an Ig domain (129-211). Clone 48A is shown to recognize CD3 /NKp46+ cells that display phenotypic and functional similarities to human NK cells. Also, it recognizes a subset of T cells that are NKp46 positive. (Ref.: Foltz, JA et al. (2016). Front. Immunol. 7; 521).

L-cells expressing canine NKp46 fusion protein

Anti-canine NKp46 (CD335), clone 48A, Cat. No. MABF2109, is a mouse monoclonal antibody that detects canine Natural cytotoxicity triggering receptor 1(NKp46) and has been tested for use in ELISA, Flow Cytometry, and Immunocytochemistry.

ELISA Analysis: A representative lot detected canine NKp46 (CD335) in ELISA applications (Foltz, J.A., et. al. (2016). Front Immunol. 7:521).

Flow Cytometry Analysis: A representative lot detected canine NKp46 (CD335) in Flow Cytometry applications (Foltz, J.A., et. al. (2016). Front Immunol. 7:521).

Clone 48A is a mouse monoclonal antibody that specifically detects canine NKp46.

Protein G purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Immunocytochemistry in canine peripheral blood mononuclear cells (PBMCs).

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected canine NKp46 (CD335) in canine peripheral blood mononuclear cells (PBMCs).

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.