Anti-HLA‑DQA1 Antibody, clone SPV-L3

HLA class II histocompatibility antigen, DQ alpha 1 chain (UniProt: P01909; also known as DC-1 alpha chain, DC-alpha, HLA-DCA, MHC class II DQA1) is encoded by the HLA-DQA1 gene (Gene ID: 3117) in human. HLA‑DQA1, a member of the MHC class II family, is a single-pass type I membrane glycoprotein, which is synthesized with a signal peptide (aa 1-23) that is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 24-216), a transmembrane domain (aa 217-239), and a cytoplasmic domain (aa 240-254). It is also detected in endoplasmic reticulum (ER), Golgi apparatus, and endosome membranes. It is a heterodimer of an and a β subunit. It is reported to bind peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. Its peptide binding cleft accommodates peptides of 10-30 residues. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules. Autophagy is also shown to be a source of endogenous peptides and autophagosomes are reported to constitutively fuse with MHC class II loading compartments. Clone SPV-L3 is shown to recognize a class II MHC products different from HLA-DR. It is reported to block the cytotoxic activity of CTL clones JR-2-19 and JR-2-26. (Ref.: Spits, H., et al. (1984). Eur. J. Immunol. 14(4); 299-304).

T3+ T4+ T8- Cytotoxic T lymphocytes clone HG-38.

Quality Control Testing

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis: 1.0 µg of this antibody detected HLA-DQA1 in one million Human peripheral blood mononuclear cells (PBMC).

Tested Applications

Immunoprecipitation Analysis: A representative lot detected HLA‑DQA1 in Immunoprecipitation applications (Spits, H., et al. (1984). Eur J Immunol. 14(4):299-304).

Blocking of Intefering Clones: A representative lot blocked the activity of CTL clones JR-2-19 and JR-2-26. (Spits, H., et al. (1984). Eur J Immunol. 14(4):299-304).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone SPV-L3 is a mouse monoclonal antibody that detects HLA class II histocompatibility antigen, DQ alpha 1 chain (HLA-DQA1).

Purified mouse monoclonal antibody IgG2a in PBS without preservatives.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.