Anti-Complement C5 Antibody, clone SIM 225-3.2.1

Complement C5 (UniProt: P01031; also known as C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4) is encoded by the C5 (also known as CPAMD4) gene (Gene ID: 727) in human. The complement system provides innate defense against microbial infection and is a complement to antibody-mediated immunity. It consists of about thirty-five interacting plasma and membrane associated proteins. Activation of the complement system is achieved through three major independent, but overlapping, pathways: the classical pathway, which is activated by certain isotypes of antibodies bound to antigens (immune complexes); the alternative pathway, which is activated on microbial cell surfaces in the absence of antibody; and the lectin pathway, which is activated by a plasma lectin (mannose binding lectin) that binds to mannose residues on microbes. The human C5 complement component is a glycoprotein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to produce the mature protein that can further cleave into four chains: Complement C5 beta chain (aa 19-673); Complement C5 alpha chain (aa 678-1676); C5a anaphylatoxin (aa 678-751); and Complement C5 alpha′ chain (aa752-1676). The C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b. C5a serves as an inflammatory mediator that can activate a variety of cells through G-protein-linked receptors. The C5b fragment combines with C6, C7, C8, and C9 to form lytic C5b-9 complement membrane attack complex (MAC) that is responsible for the membrane lytic events associated with complement activation. Mutations in C5 gene are known to cause complement component 5 deficiency (C5D), a rare defect of the complement classical pathway that leads to susceptibility to severe recurrent infections. (Ref.: Monedero, M., et al. (2019). Comp. Immunol. Microbiol. Infect. Dis. 62; 7-12; Horiuchi, T., and Tsukamoto, H. (2016). Inflamm. Regen. 36; Article 11).

Complement C5 from human serum.

Quality Control Testing

Evaluated by Western Blotting with Human Complement C5 glycoprotein (non-reduced).

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected non-reduced Human Complement C5.

Tested Applications

Western Blotting Analysis: A representative lot detected Complement C5 in Western Blotting applications (Monedero, M., et al. (2019). Comp Immunol Microbiol Infect Dis. 62:7-12).

Flow Cytometry Analysis: A representative lot detected Complement C5 in Flow Cytometry applications (Monedero, M., et al. (2019). Comp Immunol Microbiol Infect Dis. 62:7-12).

ELISA Analysis: A representative lot detected Complement C5 in applications (Monedero, M., et al. (2019). Comp Immunol Microbiol Infect Dis. 62:7-12).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone SIM 225-3.2.1 is a mouse monoclonal antibody that detects Complement 5.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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