Anti-MAdCAM-1 Antibody, clone MECA-89

Mucosal addressin cell adhesion molecule 1 (UniProt: Q61826; also known as MAdCAM-1, mMAdCAM-1) is encoded by the Madcam1 gene (Gene ID: 17123) in murine species. MAdCAM-1 is a cell adhesion leukocyte receptor that is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off in mature form that contains an extracellular domain (aa 22-109), a transmembrane domain (aa 365-385), and a cytoplasmic domain (aa 386-405). It also contains three Ig-like domains (aa 22-109; 110-227; 258-357) and a Mucin-like region (aa 221-257). It helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It is highly expressed on high endothelial venules (HEV) of organized intestinal lymphoid tissues and mesenteric lymph nodes, and in the lamina propria of the intestine. Its levels are up-regulated during gut inflammation. Its expression is also observed in brain endothelioma cells and mucosal tissues during chronic inflammation. Aberrant expression of MAdCAM-1 has also been reported in the inflamed pancreas. MAdCAM-1 can bind both the integrin 4/β7 and L-selectin and is involved in regulating both the passage and retention of leukocytes. Two isoforms of MAdCAM-1 have been described that are produced by alternative splicing. Both isoforms can adhere to integrin 4/β7. Isoform 2 that lacks the mucin-like domain is believed to be specialized in supporting integrin 4/β7-dependent adhesion strengthening, independent of L-selectin binding. Interaction between integrin 4 and MAdCAM-1 is considered to be important for the homing of diabetogenic T cells to the pancreas. (Ref.: Rivera-Nieves, J., et al. (2005). J. Immunol. 174(4); 2343 2352; Bargatze, RF., et al. (1995). Immunity. 3(1); 99-108; Streeter, PR., et al. (1988). Nature. 331(6151); 41-46).

Endothelial cells isolated from BALB/c mesenteric and peripheral lymph nodes

Quality Control Testing

Evaluated by Immunohistochemistry in Mouse colon tissue sections.

Immunohistochemistry (frozen) Analysis: A representative lot detected MAdCAM-1 in frozen sections of Mouse colon tissue.

Tested Applications

Immunohistochemistry Applications: A representative lot detected MAdCAM-1 in Immunohistochemistry applications (Streeter, P.R., et al. (1988). Nature. 331(6151):41-6; Grayson, M.H., et al. (2001). J Immunol Methods. 256(1-2):55-63).

Inhibition Assay: A representative lot inhibited L-selectin lymphocyte rolling in Peyer Patch- endothelial venules (HEV).(Bargatze, R.F., et al. (1995). Immunity. 3(1):99-108).

Immunocytochemistry Analysis: A representative lot detected MAdCAM-1 in Immunocytochemistry applications (Bachmann, C., et al. (2006). Gastroenterology. 130(1):8-16).

Flow Cytometry Analysis: A representative lot detected MAdCAM-1 in Flow Cytometry applications (Honda, K., et al. (2001). J Exp Med. 193(5):621-30).

Immunoaffinity Purification: A representative lot was used for Immunoaffinity Purification of MAdCAM-1. (Streeter, P.R., et al. (1988). Nature. 331(6151):41-6).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone MECA-89 is a rat monoclonal antibody that detects Mucosal addressin cell adhesion molecule 1 (MAdCAM-1). It targets an epitope within the extracellular domain.

Purified rat monoclonal antibody IgG2a in PBS without azide.

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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