MABN15
Anti-Rhodopsin Antibody, clone 4D2
clone 4D2, from mouse
Synonym(s):
rhodopsin, rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant), retinitis pigmentosa 4, autosomal dominant, opsin 2, rod pigment
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
4D2, monoclonal
Application:
ELISA, ICC, IHC, WB
Citations:
38
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
4D2, monoclonal
species reactivity
rat, human, fish
species reactivity (predicted by homology)
mouse (based on 100% sequence homology)
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... RHO(6010)
rat ... Rho(24717)
General description
Expected at ~ 39 kDa
Rhodopsin is a multi-pass, G-protein-coupled membrane receptor protein belonging to the G-protein coupled receptor 1 family. Found within rod photoreceptor cells, rhodopsin is activated by light photons in order for the visual transduction cascade to initialize. Rhodopsin is necessary to the forming of images in low light and for the viability of photoreceptor cells after birth. Mutations in rhodopsin expression are the most widely known cause of the human disease retinitis pigmentosa (RP) resulting in gradual loss of vision. At present, there are over 100 known rhodopsin mutations.
Immunogen
Epitope: N-terminus
Rat rod outer segments corresponding to rat Rhodopsin at the N-terminus.
Application
Anti-Rhodopsin Antibody, clone 4D2 is an antibody against Rhodopsin for use in IH, WB, IC, ELISA.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Sensory & PNS
Sensory & PNS
Western Blot Analysis: A previous lot of this antibody detected Rhodopsin by WB in goldfish retina lysate, as reported by Knight K, et al.
Immunocytochemistry: A previous lot of this antibody detected Rhodopsin by ICC in goldfish retina tissue, as reported by Knight K, et al
Immunohistochemistry Analysis: 1:500 dilution of this antibody has been shown to detect Rhodopsin in human eye tissue.
ELISA: Specificity of this antibody, from a different lot, was validated in a competitive ELISA, as reported by Laird D, et al.
Immunocytochemistry: A previous lot of this antibody detected Rhodopsin by ICC in goldfish retina tissue, as reported by Knight K, et al
Immunohistochemistry Analysis: 1:500 dilution of this antibody has been shown to detect Rhodopsin in human eye tissue.
ELISA: Specificity of this antibody, from a different lot, was validated in a competitive ELISA, as reported by Laird D, et al.
Biochem/physiol Actions
This antibody recognizes Rhodopsin at the N-terminus.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Rat eye tissue
Rat eye tissue
Evaluated by Immunohistochemistry in rat eye tissue.
Immunohistochemistry Analysis: 1:500 dilution of this antibody detected Rhodopsin in rat eye tissue.
Immunohistochemistry Analysis: 1:500 dilution of this antibody detected Rhodopsin in rat eye tissue.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Liang Jia et al.
The Journal of biological chemistry, 297(5), 101268-101268 (2021-10-04)
Biogenic amines activate G-protein-coupled receptors (GPCRs) in the central nervous system in vertebrate animals. Several biogenic amines, when excreted, stimulate trace amine-associated receptors (TAARs), a group of GPCRs in the main olfactory epithelium, and elicit innate behaviors. How TAARs recognize
Kayvan Samimi et al.
Biomedical optics express, 13(6), 3476-3492 (2022-07-06)
Photoreceptors are the key functional cell types responsible for the initiation of vision in the retina. Phototransduction involves isomerization and conversion of vitamin A compounds, known as retinoids, and their recycling through the visual cycle. We demonstrate a functional readout
Zhe Zhang et al.
The Journal of biological chemistry, 295(34), 12153-12166 (2020-07-09)
Pheromones play critical roles in habitat identification and reproductive behavior synchronization in the sea lamprey (Petromyzon marinus). The bile acid 3-keto petromyzonol sulfate (3kPZS) is a major component of the sex pheromone mixture from male sea lamprey that induces specific
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MABN15 | 04053252518706 |