Peripherin-2 (UniProt: P17810; also known as Retinal degeneration slow protein) is encoded by the PRPH2 (also known as RDS) gene in bovine species. Peripherin-2 is a disulfide-linked homodimeric, multi-pass membrane protein that may function as an adhesion molecule involved in stabilization and compaction of outer segment disks in retina and is considered to be essential for disk morphogenesis. It is exclusively expressed in outer segments. Peripherin-2 can form homo- and heteromeric protein complexes in outer segment. The core homomeric peripherin-2 unit is the non-covalent tetramer, which can also assemble into covalently linked octamers and higher-order oligomers. The homomeric peripherin-2 complexes are essential for proper outer segment morphology and architecture. Peripherin-2 is also shown to form heteromeric complexes with its homolog ROM-1. Mutations in PRPH2 gene can lead to autosomal dominant retinitis pigmentosa (adRP) And a majority of these mutations are located within the large loop domain connecting the transmembrane domain 3 and 4 (Ref.: Bohm, S et al. (2017). Scientific Reports 7, Article number: 2321).

~39 kDa observed; 38.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Epitope: C-terminus

Purified bovine whole peripherin.

Anti-Peripherin-2, clone 5H2, Cat. No. MABN2435, is a highly specific mouse monoclonal antibody that targets Peripherin-2 and has been tested in Immunofluorescence, Radioimmunoassay, and Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: A representative lot detected Peripherin-2 in Western Blotting applications (Connell, G., et. al. (1991). Proc Natl Acad Sci USA. 88(3):723-6; Zhang, Y., et. al. (2009). J Cell Sci. 122(Pt 8):1192-200).

Radioimmunoassay Analysis: A representative lot detected Peripherin-2 in Radioimmunoassay applications (Connell, G., et. al. (1991). Proc Natl Acad Sci USA. 88(3):723-6).

Immunofluorescence Analysis: A representative lot detected Peripherin-2 in Immunoflourescence applications (Zhang, Y., et. al. (2009). J Cell Sci. 122(Pt 8):1192-200).

Clone 5H2 detects Peripherin-2 in bovine and mouse retina.

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in bovine retinal tissue lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected Peripherin-2 in 10 µg of bovine retinal tissue lysate.

Concentration: Please refer to lot specific datasheet.

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