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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
1F6.1, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ARL6IP4(51329)
General description
Also known as ADP-ribosylation factor-like factor 6-interacting protein and ARL6IP4, the SR-25 protein is expressed in certain human organs and is relatively abundant in human testis and thymus. It is observed to mediate the Rac1 signaling pathway and acts as a splicing inhibitor of the Herpes simplex virus (HSVI); however, further research is needed to elucidate its mode of action.
Immunogen
GST-tagged recombinant protein corresponding to the C-terminus of human SR-25.
Application
Anti-SR-25 Antibody, clone 1F6.1 is a highly specific mouse monoclonal antibody, that targets SRp25 & has been tested in western blotting & ICC.
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected SR-25 in HeLa cells treated with Mimosine and Nocodazole.
Dylight® is a registered trademark of Thermo Fisher Scientific.
Dylight® is a registered trademark of Thermo Fisher Scientific.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
Quality
Evaluated by Western Blotting in HeLa cells treated with Mimosine and Nocodazole.
Western Blotting Analysis: 1 µg/mL of this antibody detected SR-25 in HeLa cells treated with Mimosine and Nocodazole.
Western Blotting Analysis: 1 µg/mL of this antibody detected SR-25 in HeLa cells treated with Mimosine and Nocodazole.
Target description
~32 kDa observed. Uniprot describes six isoforms between 22 kDa and 38 kDa
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
DyLight is a registered trademark of Pierce Biotechnology, Inc.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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