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Merck
CN

MABN50

Sigma-Aldrich

Anti-OLIG2 Antibody

mouse monoclonal, 211F1.1

Synonym(s):

oligodendrocyte lineage transcription factor 2, oligodendrocyte-specific bHLH transcription factor 2, human protein kinase C-binding protein RACK17, Protein kinase C-binding protein 2, protein kinase C binding protein 2, Class B basic helix-loop-helix pr

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Key Documents

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Product Name

Anti-Olig2 Antibody, clone 211F1.1, clone 211F1.1, from mouse

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

clone

211F1.1, monoclonal

species reactivity

mouse

species reactivity (predicted by homology)

rat (based on 100% sequence homology), human (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_005797

UniProt accession no.

Q13516

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... OLIG2(10215)

General description

Oligodendrocyte lineage transcription factor 2 (Olig2) is a protein belonging to group A of the OLIG family of basic helix-loop-helix transcription factors (bHLH). bHLH proteins are divided into 6 groups (A-F), and characterized by their biochemical properties and structure. Olig2 is classified as group A due to its ability to form heterodimers with other bHLH proteins and binding to the E box. Olig2 has a crucial role during development in specifying the final location of motor neurons and oligodendrocytes in the spinal cord, along with the development within the hindbrain of somatic motor neurons. Olig2 expression is observed within oligodendrocytes and in developing astrocytes. Recent studies have shown strong expression of Olig2 in human oligodendrogliomas, primary tumors within the central nervous system, and moderate expression in astrocytomas. These observations have led to the proposal of Olig2 having potential as an oligodendroglioma diagnostic marker.
~37 kDa observed

Immunogen

Epitope: Unknown
Recombinant protein corresponding to human Olig2.

Application

Detect Olig2 using this Anti-Olig2 Antibody, clone 211F1.1 validated for use in WB, IC, IH & IP.
Immunohistochemistry Analysis: This antibody has been shown to detect Olig2 in coronal brain tissue as reported by an independent laboratory (Cai, J., et al. (2007). Development. 134:1887-1899).

Immunocytochemistry Analysis: This antibody has been shown to detect Olig2, as reported by an independent laboratory (Lee, S.K., et al. (1995). Genes & Dev. 19:282-294).

Immunoprecipitation Analysis: This antibody has been shown to detect Olig2, as reported by an independent laboratory (Lee, S.K., et al. (1995). Genes & Dev. 19:282-294).
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Biochem/physiol Actions

Anti-Olig2 Antibody recognizes Olig2.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain lysate
Evaluated by Western Blot in mouse brain lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected Olig2 on 10 µg of mouse brain lysate.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Contribution of microRNA-1275 to Claudin11 protein suppression via a polycomb-mediated silencing mechanism in human glioma stem-like cells.
Katsushima, K; Shinjo, K; Natsume, A; Ohka, F; Fujii, M; Osada, H; Sekido, Y; Kondo, Y
The Journal of Biological Chemistry null
An efficient and economical culture approach for the enrichment of purified oligodendrocyte progenitor cells.
Jianqin Niu,Lingyun Wang,Shubao Liu,Chengren Li,Jiming Kong,Hai-Ying Shen,Lan Xiao
Journal of Neuroscience Methods null
Diverse behaviors of outer radial glia in developing ferret and human cortex.
Gertz, CC; Lui, JH; LaMonica, BE; Wang, X; Kriegstein, AR
The Journal of Neuroscience null
Restoration of oligodendrocyte pools in a mouse model of chronic cerebral hypoperfusion.
McQueen, J; Reimer, MM; Holland, PR; Manso, Y; McLaughlin, M; Fowler, JH; Horsburgh, K
Testing null
Michael J E Joseph et al.
Translational stroke research, 7(3), 192-208 (2016-01-09)
Damage to myelinated axons contributes to neurological deficits after acute CNS injury, including ischemic and hemorrhagic stroke. Potential treatments to promote re-myelination will require fully differentiated oligodendrocytes, but almost nothing is known about their fate following intracerebral hemorrhage (ICH). Using
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