Anti-ACE2 Antibody, clone 4G5.1

Angiotensin-converting enzyme 2 (ACE2) is a transmembrane protein belonging to the metallopeptidases (M2) family. It is a membrane-associated carboxypeptidase, expressed in the vascular endothelial cells of the heart, kidney, testis, gastrointestinal system and in the epithelial cells of lungs. ACE2 consists of an N-terminal peptidase domain (PD) and a C-terminal collectrin-like domain (CLD). It also comprises a single catalytic domain that is similar to that of ACE. The gene ACE2 is mapped to human chromosome location Xp22.2. ACE2 is a structural homolog ACE.

~62 kDa observed. Uniport describes 2 isoforms at ~92 kDa (Isoform 1) and ~64 kDa (Isoform 2) due to alternative splicing.

GST-tagged recombinant protein corresponding to human ACE2.

Anti-ACE2 Antibody, clone 4G5.1 detects level of ACE2 & has been validated for use in Western Blotting.

ELISA Analysis: A representative lot detected ACE2 in a direct ELISA.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Angiotensin-converting enzyme-2 (ACE2) produces angiotensin (1-9) by hydrolysis of angiotensin 1. Angiotensin (1-9) is a precursor for angiotensin (1-7). ACE2 may also directly produce angiotensin (1-7) by conversion of angiotensin II, which is involved in vasoconstriction.(2) Angiotensin (1-7) plays an important role in the renin-angiotensin system (RAS) signaling pathway and is involved in bradykinin-induced vasodilation. Hence, ACE2 antagonizes the effects of its homolog, ACE. ACE2 is insensitive to commonly used inhibitors such as lisinopril and captopril. This gene is upregulated in heart failure due to the overactivity of angiotensin II. ACE2 is studied for its potential therapeutic value for hypertension and heart failure. It binds to the viral envelope protein spike (S) protein and acts as a major entry receptor for severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2 in human cells. Hence, it is considered as a potential therapeutic target for COVID-19. ACE2 exhibits protection from lung injury.

This antibody recognizes ACE2.

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HEK293 cell lysate

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: A 1:2,000 dilution of this antibody detected ACE2 in 10 µg of HEK293 cell lysate.

Replaces: MAB5676

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