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MABN832

Sigma-Aldrich

Anti-pan-AMPA receptor (GluR1-4), clone 2D8 Antibody

clone 2D8, from mouse

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Synonym(s):
Glutamate receptor 1, GluR-1, AMPA-selective glutamate receptor 1, GluR-A, GluR-K1, Glutamate receptor ionotropic, AMPA 1, GluA1
eCl@ss:
32160702

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

2D8, monoclonal

species reactivity

human, rat

technique(s)

ELISA: suitable
electron microscopy: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NP_000817
NP_000818
NP_000819
NP_000820

UniProt accession no.

P42261
P42262
P42263
P48058

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GRIA1(2890)

General description

Glutamate receptors are synaptic receptors located primarily on the membranes of neuronal cells. Upon ligand stimulation, ionotropic glutamate receptors (iGluRs) form non-selective cation channels, while metabotropic glutamate receptors (mGluRs) indirectly activate ion channels via G protein-mediated signaling. AMPA receptors are iGluRs and are also known as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors, AMPARs, or quisqualate receptors. AMPARs are composed of four types of subunits, GluR1/GluA1 (UniProt P42261) is encoded by GRIA1, GluR2/GluA2 (UniProt P42262) by GRIA2, GluR3/GluA3 (UniProt P42263) by GRIA3, and GluR4/GluA4 (UniProt P48058) by GRIA4 gene in human. AMPARs are tetrameric in nature, consisting of two sets of homodimers, most often a set of GluR2 dimer is coupled with a set of GluR1, GluR3 or GluR4 homodimer. Cat. No. Anti-pan-AMPA receptor (GluR1-4), clone 2D8, reacts with all four AMPA receptor subunits.

Immunogen

Fusion protein coding for the extracellular domain of AMPA Glutamate receptor 1-4 corresponding to human pan-AMPA receptor (GluR1-4).

Application

Anti-pan-AMPA receptor (GluR1-4), clone 2D8 Antibody is an antibody against AMPA receptor for use in Western Blotting, Immunohistochemistry and Immunocytochemistry, Enzyme Immunoassay (ELISA), Electron Microscopy and Immunofluorescence.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected AMPA receptor (GluR1-4) in 10 µg of human brain tissue lysate.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected AMPA receptor (GluR1-4) in 10 µg of transfected human GluA1 HEK293, transfected rat GluA1 HEK293, transfected rat GluA2 HEK293, transfected rat GluA3 HEK293, and Mock transfected cell lysate.
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected AMPA receptor (GluR1-4) in human cerebral cortex, human thalamus, rat cerebellum, and rat cerebral cortex tissue.
Immunocytochemistry Analysis: A representative lot detected AMPA receptor (GluR1-4) in Hela cells transfected with GluA1, GluA2, GluA3 and GluA4 (Courtesy of Dr John Morrison, Mount Sinai School of Medicine).
Immunofluorescence Analysis: A representative lot detected AMPA receptor (GluR1-4) in rodent, primate and human brain sections (Courtesy of Dr John Morrison, Mount Sinai School of Medicine).
Electron Microscopy Analysis: A representative lot detected AMPA receptor (GluR1-4) in rat hippocampus (Courtesy of Dr John Morrison, Mount Sinai School of Medicine).
ELISA Analysis: A representative lot detected AMPA receptor (GluR1-4) in TrpE fusion proteins (Courtesy of Dr John Morrison, Mount Sinai School of Medicine).

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected AMPA receptor (GluR1-4) in 10 µg of rat brain tissue lysate.

Target description

~85 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

recommended

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Noemí Esteras et al.
Alzheimer's & dementia : the journal of the Alzheimer's Association, 18(2), 318-338 (2021-06-01)
The second most common form of early-onset dementia-frontotemporal dementia (FTD)-is often characterized by the aggregation of the microtubule-associated protein tau. Here we studied the mechanism of tau-induced neuronal dysfunction in neurons with the FTD-related 10+16 MAPT mutation. Live imaging, electrophysiology
Hang Zhou et al.
British journal of anaesthesia, 126(6), 1141-1156 (2021-03-02)
Both animal and retrospective human studies have linked extended and repeated general anaesthesia during early development with cognitive and behavioural deficits later in life. However, the neuronal circuit mechanisms underlying this anaesthesia-induced behavioural impairment are poorly understood. Neonatal mice were
Alessandro L Gallina et al.
Frontiers in cardiovascular medicine, 8, 655869-655869 (2021-05-08)
Objectives and Aims: Vascular smooth muscle cells (VSMCs) are key constituents of both normal arteries and atherosclerotic plaques. They have an ability to adapt to changes in the local environment by undergoing phenotypic modulation. An improved understanding of the mechanisms
Wenyan Han et al.
Frontiers in molecular neuroscience, 10, 402-402 (2017-12-26)
In the brain, AMPA receptors (AMPARs)-mediated excitatory synaptic transmission is critically regulated by the receptor auxiliary subunits. Recent proteomic studies have identified that Ferric Chelate Reductase 1 Like protein (FRRS1L), whose mutations in human lead to epilepsy, choreoathetosis, and cognitive

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