Anti-Periostin Antibody, clone 5B2.3

Periostin (UniProt Q15063; also known as OSF-2, Osteoblast-specific factor 2, PN) is encoded by the POSTN (also known as PSF2) gene (Gene ID 10631) in human. Periostin is an extracellular matrix (ECM) protein predominantly expressed in collagen-rich fibrous connective tissues, including heart valves, tendons, perichondrium, periosteum, and periodontal ligament (PDL). Periostin plays an essential role in the process of remodeling during tissue/organ development/repair and inflammation. Periostin derived from fibroblasts is shown to induce keratinocytes differentiation and proliferation. Periostin is a mediator for epidermal hyperplasia and is highly expressed in the dermis of both atopic dermatitis (AD) and psoriasis patients. Periostin level is also reported to be upregulated in patients with acute or chronic hepatitis and correlates well with serum TGF- 1 and TGF- 2 levels. The PDL-POSTN spliced isoform is predominantly expressed in PDL, it binds integrin v 3 with a higher affinity and induces strong activation of the integrin v 3-focal adhesion kinase (FAK) signaling pathway. Periostin is produced with a signal peptide (a.a. 1-21), the removal of which yields the mature protein with an N-terminal cysteine-rich EMI (a.a. 40-94) domain and four asciclin I or FAS1 domains (a.a. 97-230, 234-365, 368-492, 496-628). Alternative splicing generate several C-terminal domain variants with tissue-specific expression patterns.

Clone 5B2.3 epitope lies within a region C-terminal to the FAS1 domains. Immunogen covered region is 100% present in isoforms 1 (OSF-2OS), 2 (OSF-2p1), and 5, but partially missing in spliced isoforms 3, 4 (PDL-POSTN), 6, and 7. Clone 5B2.3 immunoreactivity toward these latter isoforms is possible, but has not been confirmed.

GST-tagged recombinant human periostin C-terminal fragment.

Detect Periostin using this mouse monoclonal Anti-Periostin, clone 5B2.3, Cat. No. MABS1183, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected periostin in human prostate cancer, breast and small intestine tissue sections.

Research Category
Signaling

Evaluated by Western Blotting in human stomach tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected periostin in 10 µg of human stomach tissue lysate.

~78-93 kDa observed. The broad band appearance is consistent with the detetion of differentially glycosylated species of one or more spliced isoforms. 90.96/87.021/84.67/81.50/88.07/81.17/78.00 kDa (mature isoform 1/2/3/4/5/6/7) and 93.31/87.02/87.25/83.85/90.42/83.53/80.36 kDa (isoform 1/2/3/4/5/6/7 pro-form) calculated. Uncharacterized bands may be observed in some lysate(s).

Format: Purified

Protein G purified.

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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