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Merck
CN

MABS1946

Sigma-Aldrich

Anti-GAPDH (P. falciparum) Antibody, clone 1.4

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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conjugate

unconjugated

antibody form

purified antibody

clone

1.4
monoclonal

purified by

using protein G

species reactivity

parasites, mouse

concentration

(Please refer to lot specific datasheet.)

technique(s)

immunofluorescence: suitable
western blot: suitable

isotype

IgG1λ

UniProt accession no.

target post-translational modification

unmodified

General description

His-tagged full length recombinant Glyceraldehyde-3-phosphate dehydrogenase from Plasmodium falciparum.
~37 kDa observed; 36.63 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Application

Immunofluorescence Analysis: A representative lot detected GAPDH (P. falciparum) in Immunofluorescence applications (Dreyer, A.M., et. al. (2012). J Immunol. 188(12):6225-37; Daubenberger, C.A., et. al. (2003). Biol Chem. 384(8):1227-37; Favuzza, P., et. al. (2016). Malar J. 15:161).

Western Blotting Analysis: A representative lot detected GAPDH (P. falciparum) in Western Blotting applications (Daubenberger, C.A., et. al. (2003). Biol Chem. 384(8):1227-37; Favuzza, P., et. al. (2016). Malar J. 15:161).

Biochem/physiol Actions

Clone 1.4 is a mouse monoclonal antibody that specifically detects Glyceraldehyde-3-phosphate dehydrogenase in Plasmodium falciparum. It targets an epitope within the N-terminal half.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in Plasmodium falciparum infected human erythrocytes.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected GAPDH in Plasmodium falciparum infected human erythrocytes.

Other Notes

Plasmodium falciparum.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Regulatory Information

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