conjugate
unconjugated
antibody form
purified antibody
clone
1.4, monoclonal
purified by
using protein G
species reactivity
parasites, mouse
concentration
(Please refer to lot specific datasheet.)
technique(s)
immunofluorescence: suitable, western blot: suitable
isotype
IgG1λ
UniProt accession no.
target post-translational modification
unmodified
General description
His-tagged full length recombinant Glyceraldehyde-3-phosphate dehydrogenase from Plasmodium falciparum.
~37 kDa observed; 36.63 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Application
Immunofluorescence Analysis: A representative lot detected GAPDH (P. falciparum) in Immunofluorescence applications (Dreyer, A.M., et. al. (2012). J Immunol. 188(12):6225-37; Daubenberger, C.A., et. al. (2003). Biol Chem. 384(8):1227-37; Favuzza, P., et. al. (2016). Malar J. 15:161).
Western Blotting Analysis: A representative lot detected GAPDH (P. falciparum) in Western Blotting applications (Daubenberger, C.A., et. al. (2003). Biol Chem. 384(8):1227-37; Favuzza, P., et. al. (2016). Malar J. 15:161).
Western Blotting Analysis: A representative lot detected GAPDH (P. falciparum) in Western Blotting applications (Daubenberger, C.A., et. al. (2003). Biol Chem. 384(8):1227-37; Favuzza, P., et. al. (2016). Malar J. 15:161).
Biochem/physiol Actions
Clone 1.4 is a mouse monoclonal antibody that specifically detects Glyceraldehyde-3-phosphate dehydrogenase in Plasmodium falciparum. It targets an epitope within the N-terminal half.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in Plasmodium falciparum infected human erythrocytes.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected GAPDH in Plasmodium falciparum infected human erythrocytes.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected GAPDH in Plasmodium falciparum infected human erythrocytes.
Other Notes
Plasmodium falciparum.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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