Cytochrome b (UniProt: P00156; also known as Complex III subunit 3, Complex III subunit III, Cytochrome b-c1 complex subunit 3, Ubiquinol-cytochrome-c reductase complex cytochrome b subunit) is encoded by the MT-CYB (also known as COB, CYTB, MTCYB) gene (Gene ID: 4519) in human. Cytochrome b is a mitochondrial inner membrane protein that is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex). It is a part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c and contributes to the generation of a proton gradient across the mitochondrial membrane, which is used for ATP synthesis. The MT-CYB gene is a good phylogenetic marker. Defects in MT-CYB gene are a rare cause of mitochondrial dysfunction underlying different myopathies, which include mitochondrial encephalomyopathy, hypertrophic cardiomyopathy, and sporadic mitochondrial myopathy. Some cases of Leber hereditary optic neuropathy (LHON) are also linked to defects in MT-CYB gene, which result in acute or subacute loss of central vision, due to optic nerve dysfunction.

~28 kDa observed; 42.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Clone 5B3-6E3 detects cytochrome b in human mitochondria. It targets an epitope within the N-terminal region.

Epitope: N-terminus

KLH-conjugated Synthetic peptide corresponding to the N-terminus of human cytochrome b.

Anti-Cytochrome b, clone 5B3-6E3, Cat. No. MABS2036, is a mouse monoclonal antibody that detects Complex III Cytochrome b and has been tested for use in Western Blotting.

Research Category
Signaling

Western Blotting Analysis: 0.1 µg/mL from a representative lot detected Cytochrome b in mitochondria from human neonatal dermal fibroblasts and mitochondria from human neonatal dermal fibroblasts depleted of mtDNA. (Courtesy of Michael F. Marusich, Ph.D., mAbDx, Inc., Eugene, OR USA)

Format: Purified

Protein L

Purified mouse monoclonal antibody IgG2b in buffer containing HEPES-Buffered Saline (150 mM NaCl, 15 mM HEPES, pH 7.2) with 0.02% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in Mitochondria from human neonatal dermal fibroblasts and mitochondria from human neonatal dermal fibroblasts depleted of mtDNA.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected Cytochrome b in Mitochondria from human neonatal dermal fibroblasts and mitochondria from human neonatal dermal fibroblasts depleted of mtDNA.

Concentration: Please refer to lot specific datasheet.

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