NFATc1, also known as Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1, is a component of the nuclear factor of activated T cells DNA-binding transcription complex which consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. This protein plays a main role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription and functions as a major molecular target for the immunosuppressive drugs such as cyclosporine A. NFATc1 is expressed in most human primary lymphocytes and mature human T- and B-cell neoplasms.

~~95, ~110 and ~130 kDa observed

Recombinant protein of human NFATc1 amino acids 197-304

Detect NFAT using this mouse monoclonal antibody, Anti-NFATc1 Antibody, clone 7A6 validated for use in western blotting, Flow Cytometry & Immunofluorescence.

Flow Cytometry Analysis: A representative lot detected human NFATc1 in Flow Cytometry. (Brandt C, et al. 2010.)

Immunofluorescent Analysis: A representative lot detected human NFATc1 in Immunofluorescence. (Timmerman LA, et al. 1997.)

Format: Purified

Purified mouse monoclonal IgG1 antibody in buffer containing PBS (pH 7.2) with up to 0.1% sodium azide.

The antibody solution should be stored undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Evaluated by Western Blotting in Jurkat, human Th1, and mouse Th1 cell extracts.

Western Blotting Analysis: 1 μg/mL of this antibody detected in Jurkat, human Th1, and mouse Th1 cell extracts.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.