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MABS441

Anti-WBP2 Antibody, clone 4C8H10

clone 4C8H10, from mouse

Synonym(s):

WW domain-binding protein 2, WBP-2

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
4C8H10, monoclonal
Application:
immunocytochemistry
immunohistochemistry
immunoprecipitation (IP)
western blot
Species reactivity:
human
Citations:
1
Technique(s):
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Uniprot accession no.:
Q969T9

Key Documents

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Product Name

Anti-WBP2 Antibody, clone 4C8H10, clone 4C8H10, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4C8H10, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_036610

UniProt accession no.

Q969T9

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... WBP2(23558)

Analysis Note

Evaluated by Western Blotting in MDA-MB-231 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected WBP2 in 10 µg of MDA-MB-231 cell lysate.

Application

Immunohistochemistry Analysis: A representative lot detected WBP2 in breast cancer tissue at 1.25 ug/ml
Immunocytochemistry Analysis: A representative lot detected WBP2 in transfected HeLa cell fixed with 4% paraformaldehyde at 20 ug/ml
Immunoprecipitation Analysis: A representative lot immunoprecipitated transfected V5-WBP2 from HeLa cell
Research Category
Signaling

Apoptosis & Cancer
Research Sub Category
Growth Factors & Receptors
This Anti-WBP2 Antibody, clone 4C8H10 is validated for use in Western Blotting and Immunoprecipitation and Immunocytochemistry and Immunohistochemistry for the detection of WBP2.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

WBP2 is a protein-protein interacting protein that binds to the WW domain of YAP1, WWP1 and WWP2 proteins. WW domain-containing proteins are found in most cells and play important roles in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. They are also upregulated in many cancers. WBP2 also interacts with the ubiquitin ligase proteins belonging to the NEDD4 family and appears to assist in the regulation of targeting proteins to proteasomes or lysosomes. Also in WBP2 knockout mice, the mice display abnormal brainstem and hearing development, indicating an important role in development as well.
~ 37 kDa observed

Immunogen

GST-tagged full length recombinant protein corresponding to Human WBP2.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Zilin Li et al.
The Journal of biological chemistry, 293(52), 20014-20028 (2018-11-18)
The transcriptional coactivator WW domain-binding protein 2 (WBP2) is an emerging oncogene and serves as a node between the signaling protein Wnt and other signaling molecules and pathways, including epidermal growth factor receptor, estrogen receptor/progesterone receptor, and the Hippo pathway.

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