Skip to Content
Merck
CN

Skip To

MABS465

Sigma-Aldrich

Anti-Nampt, clone 14A.5 Antibody

clone 14A5, from rat

Synonym(s):

PBEF, Nicotinamide phosphoribosyltransferase, NAmPRTase, Nampt, Pre-B-cell colony-enhancing factor 1 homolog, PBEF, Visfatin

Sign Into View Organizational & Contract Pricing

Select a Size

100 μG
CN¥4,489.38

CN¥4,489.38


Estimated to ship onAugust 11, 2025Details

Promotion available. Please contact an authorized dealer to order.

Request a Bulk Order

Select a Size

Change View
100 μG
CN¥4,489.38

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

CN¥4,489.38


Estimated to ship onAugust 11, 2025Details

Promotion available. Please contact an authorized dealer to order.

Request a Bulk Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

rat

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

14A5, monoclonal

species reactivity

human, mouse

technique(s)

ELISA: suitable
immunohistochemistry: suitable
inhibition assay: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
HPA047776AV42254MABS528
Gene Information

human ... NAMPT(10135)

Gene Information

human ... NAMPT(10135)

Gene Information

human ... PBEF1(10135)

Gene Information

human ... HPRT1(3251)

biological source

rat

biological source

rabbit

biological source

rabbit

biological source

mouse

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

purified antibody

species reactivity

human, mouse

species reactivity

human

species reactivity

rat, rabbit, horse, bovine, mouse, human, dog, guinea pig

species reactivity

human

clone

14A5, monoclonal

clone

polyclonal

clone

polyclonal

clone

13H11.1, monoclonal

technique(s)

ELISA: suitable, inhibition assay: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunohistochemistry: 1:200-1:500

technique(s)

western blot: suitable

technique(s)

western blot: suitable

General description

Nicotinamide phosphoribosyltransferase (NAMPT) is a member of the NAPRTase family. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. NAMPT is localized in the cytoplasm and expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis.
~56 kDa observed

Immunogen

Recombinant protein corresponding to mouse Nampt.

Application

Anti-Nampt antibody, clone 14A.5 is an antibody against Nampt for use in western blotting, IHC, inhibition & ELISA.
Western Blotting Analysis: 0.1 µg/mL from a representative lot detected Nampt in Anti-CD3epsilon stimulated mouse spleen cell lysate (Prof. Oberdan Leo, Universite Libre de Bruxelles.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Nampt in mouse spleen tissue lysate (Ronveaux, A., et al. (2002). Eur J Immunol. 32(11):3225-3234.).

Inhibition Analysis: A representative lot from an independent laboratory was inhibited by a synthetic peptide corresponding to murine Nampt in total liver extracts (Ronveaux, A., et al. (2002). Eur J Immunol. 32(11):3225-3234.).

Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected Nampt in CD11c positive mouse spleen sections (Prof. Oberdan Leo, Universite Libre de Bruxelles.).

ELISA Analysis: A representative lot from an independent laboratory detected recombinant Nampt peptides (Ronveaux, A., et al. (2002). Eur J Immunol. 32(11):3225-3234.).

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Nampt in 10 µg of Raji cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


  • Certificates of Analysis (COA)

    Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

    Select a Product Number

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Arnau Llobet Rosell et al.
    eLife, 11 (2022-12-09)
    Axon degeneration contributes to the disruption of neuronal circuit function in diseased and injured nervous systems. Severed axons degenerate following the activation of an evolutionarily conserved signaling pathway, which culminates in the activation of SARM1 in mammals to execute the
    Uffe H Olesen et al.
    BMC cancer, 10, 677-677 (2010-12-15)
    Inhibitors of nicotinamide phosphoribosyltransferase (NAMPT) are promising cancer drugs currently in clinical trials in oncology, including APO866, CHS-828 and the CHS-828 prodrug EB1627/GMX1777, but cancer cell resistance to these drugs has not been studied in detail. Here, we introduce an

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service