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MABS465

Anti-Nampt, clone 14A.5 Antibody

clone 14A5, from rat

Synonym(s):

PBEF, Nicotinamide phosphoribosyltransferase, NAmPRTase, Nampt, Pre-B-cell colony-enhancing factor 1 homolog, PBEF, Visfatin

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
14A5, monoclonal
Application:
ELISA
immunohistochemistry
inhibition assay
western blot
Species reactivity:
human, mouse
Citations:
3
Technique(s):
ELISA: suitable
immunohistochemistry: suitable
inhibition assay: suitable
western blot: suitable
Uniprot accession no.:
Q99KQ4

Key Documents

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Product Name

Anti-Nampt, clone 14A.5 Antibody, clone 14A5, from rat

biological source

rat

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

14A5, monoclonal

species reactivity

human, mouse

technique(s)

ELISA: suitable
immunohistochemistry: suitable
inhibition assay: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_067499

UniProt accession no.

Q99KQ4

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... NAMPT(10135)

Analysis Note

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Nampt in 10 µg of Raji cell lysate.

Application

Anti-Nampt antibody, clone 14A.5 is an antibody against Nampt for use in western blotting, IHC, inhibition & ELISA.
Western Blotting Analysis: 0.1 µg/mL from a representative lot detected Nampt in Anti-CD3epsilon stimulated mouse spleen cell lysate (Prof. Oberdan Leo, Universite Libre de Bruxelles.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Nampt in mouse spleen tissue lysate (Ronveaux, A., et al. (2002). Eur J Immunol. 32(11):3225-3234.).

Inhibition Analysis: A representative lot from an independent laboratory was inhibited by a synthetic peptide corresponding to murine Nampt in total liver extracts (Ronveaux, A., et al. (2002). Eur J Immunol. 32(11):3225-3234.).

Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected Nampt in CD11c positive mouse spleen sections (Prof. Oberdan Leo, Universite Libre de Bruxelles.).

ELISA Analysis: A representative lot from an independent laboratory detected recombinant Nampt peptides (Ronveaux, A., et al. (2002). Eur J Immunol. 32(11):3225-3234.).

General description

Nicotinamide phosphoribosyltransferase (NAMPT) is a member of the NAPRTase family. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. NAMPT is localized in the cytoplasm and expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis.
~56 kDa observed

Immunogen

Recombinant protein corresponding to mouse Nampt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Arnau Llobet Rosell et al.
eLife, 11 (2022-12-09)
Axon degeneration contributes to the disruption of neuronal circuit function in diseased and injured nervous systems. Severed axons degenerate following the activation of an evolutionarily conserved signaling pathway, which culminates in the activation of SARM1 in mammals to execute the
Uffe H Olesen et al.
BMC cancer, 10, 677-677 (2010-12-15)
Inhibitors of nicotinamide phosphoribosyltransferase (NAMPT) are promising cancer drugs currently in clinical trials in oncology, including APO866, CHS-828 and the CHS-828 prodrug EB1627/GMX1777, but cancer cell resistance to these drugs has not been studied in detail. Here, we introduce an

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