The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. The 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade. For this reason, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase. The ERK1/2 signaling cascade has been shown to be a critical regulator of cell differentiation, cell physiology and neuronal function. Aberrant control of ERK1/2 activity has been implicated in a variety of pathological conditions, including cancer and autoimmune diseases, and efficient study methods are in demand. Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.

Antibody recognizes dual phosphorylation of MAPK1/2 at Thr203/Tyr204, but this antibody has not been used for the detection of single phosphorylation at either site.

KLH-conjugated linear peptide corresponding to human MAPK 1/2 phosphorylated at Thr203 and Ty205.

Anti-phospho-MAPK 1/2 (Thr203/Ty205) Antibody, clone 14B9.1 is a highly specific mouse monoclonal antibody & that targets MAPK/Erk1/2 & has been tested in western blotting.

Research Category
Signaling

Research Sub Category
Developmental Signaling

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected phospho-MAPK 1/2 (Thr203/Ty205) in 200 µg of A431cell lysate and EGF treated A431 cell lysate.

~ 44 kDa observed

Replaces: 05-481

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
A431cell lysate and EGF treated A431 cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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