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Merck
CN

MABS813

Anti-MAP2K2/MEK2 Antibody

mouse monoclonal, 7F5

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Species reactivity:
mouse, rat, human
Clone:
7F5, monoclonal
Application:
FACS, ICC, WB
Citations:
-
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Product Name

Anti-MAP2K2, clone 7F5 Antibody,

antibody form

ascites fluid

Quality Segment

clone

7F5, monoclonal

purified by

(unpurified)

species reactivity

mouse, rat, human

concentration

(Please refer to lot specific datasheet.)

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, western blot: suitable

isotype

IgG1

UniProt accession no.

General description

Purified recombinant fragment of human MAP2K2 expressed in E. Coli.
~48 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Application

Immunocytochemistry Analysis (ICC): A 1:200-1,000 dilution from a representative lot detected MAP2K2 in HeLa cells.

Flow Cytometry Analysis (FC): A 1:200-400 dilution from a representative lot detected MAP2K2 in HeLa cells.

Physical form

Mouse monoclonal IgG1ascitic fluid containing 0.03% sodium azide.

Preparation Note

Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Evaluated by Western Blotting in PC-12, Jurkat, HeLa and NIH/3T3 cell lysates.

Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected MAP2K2 in PC-12, Jurkat, HeLa and NIH/3T3 cell lysates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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Storage Class

10 - Combustible liquids

wgk

WGK 1



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