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MABT124

Anti-Leukemia-associated RhoGEF/LARG Antibody, clone 13D2.1

clone 13D2.1, from mouse

Synonym(s):

Rho guanine nucleotide exchange factor 12, Leukemia-associated RhoGEF

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-Leukemia-associated RhoGEF/LARG Antibody, clone 13D2.1, clone 13D2.1, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

13D2.1, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NP_056128

UniProt accession no.

Q9NZN5

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... ARHGEF12(23365)

Analysis Note

Evaluated by Western Blotting in HUVEC cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Leukemia-associated RhoGEF/LARG in 200 µg of HUVEC cell lysate.

Application

Anti-Leukemia-associated RhoGEF/LARG Antibody, clone 13D2.1 is a highly specific mouse monoclonal antibody, that targets Leukemia-associated RhoGEF & has been tested in western blotting & ICC.
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Leukemia-associated RhoGEF/LARG in HepG2 and HeLa cells.
Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The protein named Leukemia-associated RhoGEF (LARG) or alternatively known as Leukemia-associated RhoGEF protein, and encoded by the gene ARHGEF12/KIAA0382/LARG, plays a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) proteins. Leukemia-associated RhoGEF (LARG) acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. Additionally, Leukemia-associated RhoGEF (LARG) interacts with RHOA, PLXNB1 and PLXNB2, and it interacts with IGF1R beta subunit and GCSAM proteins. Leukemia-associated RhoGEF (LARG) is localized to the cytoplasm and membrane. The protein translocates to the membrane upon stimulation. Leukemia-associated RhoGEF (LARG) widely expressed with isoform specific isoforms being found in certain tissues only, such as isoform 2 is primarily found in the intestine and testis.
~ 173 kDa observed. Uncharacterized band(s) may appear in some lysates.

Immunogen

Epitope: RGSL Domain
GST-tagged recombinant protein corresponding to the RGSL domain of human Leukemia-associated RhoGEF/LARG.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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