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Merck
CN

MABT1321

Sigma-Aldrich

Anti-TRF1 Antibody, clone 572C

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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conjugate

unconjugated

antibody form

purified antibody

clone

572C
monoclonal

purified by

using protein G

species reactivity

mouse, rat

concentration

(Please refer to lot specific datasheet.)

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable

isotype

IgG1κ

UniProt accession no.

target post-translational modification

unmodified

General description

His-tagged full-length recombinant mouse Telomeric repeat-binding factor 1 (TRF1).
~51 kDa observed; 48.22 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Application

Flow Cytometry Analysis: 1 µg from a representative lot detected TRF1 in mouse embryonic fibroblasts.

Western Blotting Analysis: A representative lot detected TRF1 in Western Blotting applications (Mendez-Pertuz, M., et. al. (2017). Nat Commun. 8(1):1278).

Immunofluorescence Analysis: A representative lot detected TRF1 in Immunofluorecence applications (Mendez-Pertuz, M., et. al. (2017). Nat Commun. 8(1):1278).

Immunocytochemistry Analysis: A representative lot detected TRF1 in Immunocytochemistry applications (Mendez-Pertuz, M., et. al. (2017). Nat Commun. 8(1):1278).

Biochem/physiol Actions

Clone 572C is a rat monoclonal antibody that specifically detects murine Telomeric repeat-binding factor 1 (TRF1).

Physical form

Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in mouse embryonic fibroblast cell lysates.

Western Blotting Analysis: 4 µg/mL of this antibody detected TRF1 in mouse embryonic fibroblast cell lysates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Regulatory Information

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