conjugate
unconjugated
antibody form
purified antibody
clone
572C, monoclonal
purified by
using protein G
species reactivity
mouse, rat
concentration
(Please refer to lot specific datasheet.)
technique(s)
flow cytometry: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable
isotype
IgG1κ
UniProt accession no.
target post-translational modification
unmodified
General description
His-tagged full-length recombinant mouse Telomeric repeat-binding factor 1 (TRF1).
~51 kDa observed; 48.22 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Application
Flow Cytometry Analysis: 1 µg from a representative lot detected TRF1 in mouse embryonic fibroblasts.
Western Blotting Analysis: A representative lot detected TRF1 in Western Blotting applications (Mendez-Pertuz, M., et. al. (2017). Nat Commun. 8(1):1278).
Immunofluorescence Analysis: A representative lot detected TRF1 in Immunofluorecence applications (Mendez-Pertuz, M., et. al. (2017). Nat Commun. 8(1):1278).
Immunocytochemistry Analysis: A representative lot detected TRF1 in Immunocytochemistry applications (Mendez-Pertuz, M., et. al. (2017). Nat Commun. 8(1):1278).
Western Blotting Analysis: A representative lot detected TRF1 in Western Blotting applications (Mendez-Pertuz, M., et. al. (2017). Nat Commun. 8(1):1278).
Immunofluorescence Analysis: A representative lot detected TRF1 in Immunofluorecence applications (Mendez-Pertuz, M., et. al. (2017). Nat Commun. 8(1):1278).
Immunocytochemistry Analysis: A representative lot detected TRF1 in Immunocytochemistry applications (Mendez-Pertuz, M., et. al. (2017). Nat Commun. 8(1):1278).
Biochem/physiol Actions
Clone 572C is a rat monoclonal antibody that specifically detects murine Telomeric repeat-binding factor 1 (TRF1).
Physical form
Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in mouse embryonic fibroblast cell lysates.
Western Blotting Analysis: 4 µg/mL of this antibody detected TRF1 in mouse embryonic fibroblast cell lysates.
Western Blotting Analysis: 4 µg/mL of this antibody detected TRF1 in mouse embryonic fibroblast cell lysates.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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