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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43
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biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

3B5, monoclonal

mol wt

calculated mol wt 42.24 kDa
observed mol wt ~51 kDa

purified by

using protein G

species reactivity

human, mouse

packaging

antibody small pack of 100 μg

technique(s)

ELISA: suitable
western blot: suitable

isotype

IgG1κ

epitope sequence

N-terminal half

Protein ID accession no.

NP_060692

UniProt accession no.

Q9NVD7

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... PARVA(55742)

General description

-Parvin (UniProt: Q9NVD7; also known as Actopaxin, CH-ILKBP, Calponin-like integrin-linked kinase-binding protein, Matrix-remodeling-associated protein 2) is encoded by the PARVA (also known as MXRA2) gene (Gene ID: 55742) in human. -Parvin is a widely e

Specificity

Clone 3B5 is a mouse monoclonal antibody that detects a-Parvin (PARVA). It targets an epitope within the N-terminal half.

Immunogen

GST-tagged recombinant fragment corresponding to 344 amino acids from human -Parvin (PARVA).

Application

Quality Control Testing

Evaluated by Western Blotting in lysate from HEK293T cells overexpressing -Parvin (PARVA).

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected -Parvin in lysate from HEK293T cells overexpressing -Parvin (PARVA).

Tested Applications

Western Blotting Analysis: A representative lot detected -Parvin/PARVA in Western Blotting applications (Tu, Y., et al. (2001). J Cell Biol.;153(3):585-98; Zhang, Y., et al. (2004). J Biol. Chem. 279(40):41695-705).

ELISA Analysis: A representative lot detected -Parvin/PARVA in ELISA applications (Tu, Y., et al. (2001). J Cell Biol. 153(3):585-98).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti- -Parvin/PARVA, clone 3B5, Cat. No. MABT1440, is a mouse monoclonal antibody that detects -Parvin and is tested for use in ELISA and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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