MABT1517
Anti-Osteopontin Antibody, clone 2A1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
conjugate
unconjugated
antibody form
purified antibody
clone
2A1
monoclonal
purified by
using protein G
species reactivity
human, mouse, mouse
concentration
(Please refer to lot specific datasheet.)
technique(s)
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
capacity
immunoaffinity
UniProt accession no.
target post-translational modification
unmodified
General description
His-tagged full-length recombinant human Osteopontin.
~26 kDa observed; 35.42 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Application
ELISA Analysis: A representative lot detected Osteopontin in ELISA applications (Kazanecki, C.C., et. al. (2007). J Cell Biochem. 102(4):925-35).
Western Blotting Analysis: A representative lot detected Osteopontin in Western Blotting applications (Kazanecki, C.C., et. al. (2007). J Cell Biochem. 102(4):925-35; Baliga, S.S., et. al. (2011). PLoS One. 6(1):e14568).
Immunoaffinity Purification Analysis: A representative lot detected Osteopontin in Immunoaffinity applications (Wang, K.X., et. al. (2009). J Immunol. 182(4):2485-91).
Immunohistochemistry Analysis (IHC): A representative lot of this antibody detected Osteopontin in the brain tissue of rat. (Baliga, SS et al. (2011). PLoS One 6(1); e14568).
Immunoprecipitation Analysis (IP): A representative lot of this antibody was used in protein G immunoprecipitation of osteopontin. (Kazanecki, CC., et al, (2007). J Cell Biochem. 102(4); 925-935).
Western Blotting Analysis: A representative lot detected Osteopontin in Western Blotting applications (Kazanecki, C.C., et. al. (2007). J Cell Biochem. 102(4):925-35; Baliga, S.S., et. al. (2011). PLoS One. 6(1):e14568).
Immunoaffinity Purification Analysis: A representative lot detected Osteopontin in Immunoaffinity applications (Wang, K.X., et. al. (2009). J Immunol. 182(4):2485-91).
Immunohistochemistry Analysis (IHC): A representative lot of this antibody detected Osteopontin in the brain tissue of rat. (Baliga, SS et al. (2011). PLoS One 6(1); e14568).
Immunoprecipitation Analysis (IP): A representative lot of this antibody was used in protein G immunoprecipitation of osteopontin. (Kazanecki, CC., et al, (2007). J Cell Biochem. 102(4); 925-935).
Biochem/physiol Actions
Clone 2A1 is a mouse monoclonal antibody that specifically detects Osteopontin. It targets an epitope within the C-terminal half.
Physical form
Purified mouse monoclonal antibody IgG1 in PBS without preservatives.
Preparation Note
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in mouse hippocampus tissue lysates.
Western Blotting Analysis: 2 µg/mL of this antibody detected Osteopontin in mouse hippocampus tissue lysates.
Western Blotting Analysis: 2 µg/mL of this antibody detected Osteopontin in mouse hippocampus tissue lysates.
Other Notes
Human, Mouse.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Regulatory Information
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