Anti-Osteopontin Antibody, clone 2A1

His-tagged full-length recombinant human Osteopontin.

~26 kDa observed; 35.42 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

ELISA Analysis: A representative lot detected Osteopontin in ELISA applications (Kazanecki, C.C., et. al. (2007). J Cell Biochem. 102(4):925-35).

Western Blotting Analysis: A representative lot detected Osteopontin in Western Blotting applications (Kazanecki, C.C., et. al. (2007). J Cell Biochem. 102(4):925-35; Baliga, S.S., et. al. (2011). PLoS One. 6(1):e14568).

Immunoaffinity Purification Analysis: A representative lot detected Osteopontin in Immunoaffinity applications (Wang, K.X., et. al. (2009). J Immunol. 182(4):2485-91).

Immunohistochemistry Analysis (IHC): A representative lot of this antibody detected Osteopontin in the brain tissue of rat. (Baliga, SS et al. (2011). PLoS One 6(1); e14568).

Immunoprecipitation Analysis (IP): A representative lot of this antibody was used in protein G immunoprecipitation of osteopontin. (Kazanecki, CC., et al, (2007). J Cell Biochem. 102(4); 925-935).

Clone 2A1 is a mouse monoclonal antibody that specifically detects Osteopontin. It targets an epitope within the C-terminal half.

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Western Blotting in mouse hippocampus tissue lysates.

Western Blotting Analysis: 2 µg/mL of this antibody detected Osteopontin in mouse hippocampus tissue lysates.

Human, Mouse.

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