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Merck
CN

MABT1530

Sigma-Aldrich

Anti-Cytokeratin HMW Antibody, clone 34betaE12

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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conjugate

unconjugated

antibody form

purified antibody

clone

34betaE12
monoclonal

purified by

using protein G

species reactivity

mouse, human, human

concentration

(Please refer to lot specific datasheet.)

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

UniProt accession no.

target post-translational modification

unmodified

General description

66.04, 62.38, 58.83, and 51.56 kDa calculated for Cytokeratin 1, 5, 10, and 14, respectively.
Solubilized keratin protein derived from human stratum corneum.

Application

Immunohistochemistry (Paraffin) Analysis: A representative lot detected Cytokeratin HMW in Immunohistochemistry applications (Varma, M., et. al. (2003). Histopathology. 42(2):167-72; Gown, A.M., et. al. (1984). Am J Pathol. 114(2):309-21; Cimpean, A.M., et. al. (1999) Rom J Morphol Embryol. 45:153-7).

Western Blotting Analysis: A representative lot detected Cytokeratin HMW in Western Blotting applications (Gown, A.M., et. al. (1984). Am J Pathol. 114(2):309-21).

Biochem/physiol Actions

Clone 34betaE12 is a mouse monoclonal antibody that detects human cytokeratin.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Immunohistochemistry (Paraffin) in human skin tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected Cytokeratin HMW in human skin tissue sections.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Regulatory Information

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